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多瘤病毒中T抗原转化细胞中的磷脂酰肌醇代谢

Phosphatidylinositol metabolism in cells transformed by polyomavirus middle T antigen.

作者信息

Ulug E T, Hawkins P T, Hanley M R, Courtneidge S A

机构信息

Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

出版信息

J Virol. 1990 Aug;64(8):3895-904. doi: 10.1128/JVI.64.8.3895-3904.1990.

DOI:10.1128/JVI.64.8.3895-3904.1990
PMID:2164601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249685/
Abstract

Associated with the middle T antigen of polyomavirus is a novel phosphatidylinositol (PtdIns) kinase activity which phosphorylates PtdIns at the D-3 position of the inositol ring. We have undertaken an analysis of myo-[3H]inositol-containing compounds in a panel of NIH 3T3 cell lines stably transfected with transforming and nontransforming middle T antigen mutants. All cell lines from which PtdIns 3-kinase activity coprecipitated with middle T antigen exhibited modestly elevated levels of PtdIns(3)P and compounds with predicted PtdIns(3,4)P2 and PtdIns(3,4,5)P3 structures. Complex formation between middle T antigen and PtdIns 3-kinase correlated not with an increase in total inositol phosphate levels but rather with elevated levels of InsP2 and InsP4. A specific increase in the level of an InsP2 species which comigrated in high-pressure liquid chromatography analysis with Ins(3,4)P2 was observed. These results suggest that association of the polyomavirus middle T antigen with PtdIns 3-kinase activates a distinct inositol metabolic pathway.

摘要

多瘤病毒的中间T抗原与一种新型磷脂酰肌醇（PtdIns）激酶活性相关，该活性可使肌醇环D-3位的PtdIns磷酸化。我们对一组稳定转染了转化型和非转化型中间T抗原突变体的NIH 3T3细胞系中含肌醇-[3H]的化合物进行了分析。所有PtdIns 3激酶活性与中间T抗原共沉淀的细胞系，其PtdIns(3)P以及具有预测的PtdIns(3,4)P2和PtdIns(3,4,5)P3结构的化合物水平均适度升高。中间T抗原与PtdIns 3激酶之间的复合物形成与总肌醇磷酸水平的增加无关，而是与InsP2和InsP4水平的升高有关。在高压液相色谱分析中观察到一种与Ins(3,4)P2共迁移的InsP2种类水平有特异性增加。这些结果表明，多瘤病毒中间T抗原与PtdIns 3激酶的结合激活了一条独特的肌醇代谢途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc78/249685/b2a3714ca99e/jvirol00063-0346-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc78/249685/b2a3714ca99e/jvirol00063-0346-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc78/249685/b2a3714ca99e/jvirol00063-0346-a.jpg

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