Savolainen K M, Hirvonen M R, Tarhanen J, Nelson S R, Samson F E, Pazdernik T L
Department of Pharmacology, University of Kansas Medical Center, Kansas City 66103.
Neurochem Res. 1990 May;15(5):541-5. doi: 10.1007/BF00966214.
LiCl-induced (5 mEq/kg) regional differences in the cerebral phosphoinositide (PI) cycle were studied by measuring inositol-1-phosphate (Ins-1-P), an intermediate in the PI cycle, in male Sprague Dawley and Han/Wistar rats by gas chromatography/mass spectrometry. Control Ins-1-P levels were higher frontally than caudally in both rat strains. LiCl increased Ins-1-P levels 1.8 to 7.4 fold in different regions of brain of Sprague Dawley rats but only 1.2 to 1.8 fold in Han/Wistar rats. This strain difference offers a way to compare the effects of lithium on PI metabolism versus receptor-G protein-phospholipase C coupling mechanisms.
通过气相色谱/质谱法测量雄性斯普拉格-道利大鼠和汉/威斯塔大鼠脑中磷脂酰肌醇（PI）循环中间体肌醇-1-磷酸（Ins-1-P），研究了氯化锂（5 毫当量/千克）诱导的脑PI循环区域差异。在两种大鼠品系中，对照组Ins-1-P水平额叶均高于尾状核。氯化锂使斯普拉格-道利大鼠脑不同区域的Ins-1-P水平升高1.8至7.4倍，但在汉/威斯塔大鼠中仅升高1.2至1.8倍。这种品系差异为比较锂对PI代谢与受体-G蛋白-磷脂酶C偶联机制的影响提供了一种方法。
