Institute Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, No. 198, Gongyebei Road, Jinan 250100, China.
Appl Biochem Biotechnol. 2011 Oct;165(3-4):802-13. doi: 10.1007/s12010-011-9298-8. Epub 2011 Jun 7.
The 7S and 11S globulins from soybean proteins using reverse micelle and aqueous buffer extraction methods were characterized by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscope (SEM), and their amino acid compositions were also evaluated. SDS-PAGE did not show electrophoretic differences between 7S and 11S globulin subunits with two extraction methods. SEM analysis showed that the AOT reverse micelle processing of 7S and 11S globulins induced a reduction of droplet size. Some individual amino acid contents of 7S and 11S globulins using two extraction methods were different, some were similar. In all the samples, the glutamic acid, aspartic acid, and leucine were the dominant amino acids while the cystine and methionine were the first-limiting amino acids. The proportion of essential amino acids to the total amino acids (E/T) of the 7S globulin from aqueous buffer and reverse micelles was similar. While significant differences were obtained in the proportion of E/T of the 11S globulin.
采用反胶束和水相缓冲液提取方法，对大豆蛋白中的 7S 和 11S 球蛋白进行了特征描述，方法为十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和扫描电子显微镜（SEM），并对其氨基酸组成进行了评估。两种提取方法的 SDS-PAGE 并未显示 7S 和 11S 球蛋白亚基之间的电泳差异。SEM 分析表明，AOT 反胶束处理 7S 和 11S 球蛋白会导致液滴尺寸减小。两种提取方法得到的 7S 和 11S 球蛋白的一些个别氨基酸含量不同，有些则相似。在所有样品中，谷氨酸、天冬氨酸和亮氨酸是主要的氨基酸，而半胱氨酸和蛋氨酸是第一限制氨基酸。水相缓冲液和反胶束中的 7S 球蛋白的必需氨基酸与总氨基酸（E/T）的比例相似。而 11S 球蛋白的 E/T 比例则存在显著差异。
