人脑肿瘤体液免疫反应的检测：微量细胞毒性试验的特异性和可靠性

Detection of humoral immune response to human brain tumors: specificity and reliability of microcytotoxicity assay.

作者信息

Wood W C, Kornblith P L, Quindlen E A, Pollock L A

出版信息

Cancer. 1979 Jan;43(1):86-90. doi: 10.1002/1097-0142(197901)43:1<86::aid-cncr2820430113>3.0.co;2-g.

DOI:10.1002/1097-0142(197901)43:1<86::aid-cncr2820430113>3.0.co;2-g

Abstract

A humoral microcytotoxicity assay has been used to study the immune response to human malignant astrocytic tumors. Two series are described. When the assay was performed under uniform, ideal conditions, the sera from 36 of 45 patients with these tumors (80%) produced significant cytotoxicity against allogeneic astrocytoma cells. Only 4 of 55 (7%) normal sera tested demonstrated cytotoxicity. The second series describes a five year study of over 400 sera with several sera assayed each week. The assay was found to vary in specificity and reliability over this five year period. The reasons for this variability are discussed and methods of standardizing the technique are proposed. Recent information concerning the mechanisms of the humoral microcytotoxicity assay offer promise of improved reliability and clinical utility.

摘要

一种体液微细胞毒性测定法已被用于研究对人类恶性星形细胞瘤的免疫反应。描述了两个系列。当在统一的理想条件下进行该测定时，45例患有这些肿瘤的患者中有36例（80%）的血清对同种异体星形细胞瘤细胞产生了显著的细胞毒性。在检测的55份正常血清中，只有4份（7%）显示出细胞毒性。第二个系列描述了一项对400多份血清进行的为期五年的研究，每周检测几份血清。发现在这五年期间该测定的特异性和可靠性有所变化。讨论了这种变异性的原因，并提出了使该技术标准化的方法。关于体液微细胞毒性测定机制的最新信息有望提高其可靠性和临床实用性。