Coakham H B, Kornblith P L, Quindlen E A, Pollock L A, Wood W C, Hartnett L C
J Natl Cancer Inst. 1980 Feb;64(2):223-33. doi: 10.1093/jnci/64.2.223.
In 25 patients with intracranial gliomas, the autologous humoral response was studied in vitro with the use of microcytotoxicity (MC) and immune adherence (IA) assays. Antibodies were detected to autologous cultures in 44% of the cases by MC and in 50% by IA. These positive responses occurred in statistically different groups of patients, which suggested that different functional types of antibody were involved. Direct testing and absorption experiments showed that antibody was not significantly directed against autologous fibroblasts. Autologous cytotoxic antibodies were detected by 67% of astrocytoma cases and in only 10% of patients harboring a glioblastoma, the most anaplastic tumor of the glioma series. By means of the IA assay, absorption experiments were performed with the use of adult and fetal brains and cultures of autologous and allogeneic gliomas and fibroblasts. In this serologic system, the types of antigenic expression of a human glioma could be categorized as follows: 1) highly restricted glioma antigen(s), 2) common glioma antigen(s), 3) neurectoderm-derived antigen(s), and 4) brain and fibroblast-associated oncofetal antigen(s). The common glioma antigen and oncofetal antigen appeared to be qualitatively different, and the glioma antigen was expressed in uncultured tumor tissue.
在25例颅内胶质瘤患者中，采用微细胞毒性（MC）和免疫黏附（IA）试验对自体体液反应进行了体外研究。通过MC检测，44%的病例中检测到针对自体培养物的抗体，通过IA检测则为50%。这些阳性反应出现在统计学上不同的患者组中，这表明涉及不同功能类型的抗体。直接检测和吸收实验表明，抗体并非显著针对自体成纤维细胞。67%的星形细胞瘤病例检测到自体细胞毒性抗体，而在胶质瘤系列中最间变的肿瘤——胶质母细胞瘤患者中，只有10%检测到。通过IA试验，利用成人和胎儿脑以及自体和异体胶质瘤及成纤维细胞培养物进行了吸收实验。在这个血清学系统中，人类胶质瘤的抗原表达类型可分为以下几类：1）高度受限的胶质瘤抗原，2）常见的胶质瘤抗原，3）神经外胚层衍生抗原，4）脑和成纤维细胞相关的癌胚抗原。常见的胶质瘤抗原和癌胚抗原在性质上似乎不同，且胶质瘤抗原在未培养肿瘤组织中表达。
