Hartfield P J, Robinson J M
Department of Cell Biology, Neurobiology and Anatomy, Ohio State University, Columbus 43210.
Biochem Biophys Res Commun. 1990 Jul 16;170(1):194-200. doi: 10.1016/0006-291x(90)91259-u.
The present study utilizes an electropermeabilized cell system to determine the effect of Mg.ATP on neutrophil superoxide (O2-)-generating responses stimulated by suboptimal concentrations of fMLP, GTP gamma S and PMA. Permeabilization in the presence of exogenously added Mg.ATP was neither sufficient to initiate O2- release nor necessary for stimulated O2- production. However, the inclusion of Mg.ATP in the permeabilization medium primed the O2(-)-generating responses mediated by suboptimal concentrations of these stimuli. The site of action of Mg.ATP is intracellular. Moreover, the fact that Mg.ATP primes responses stimulated by fMLP, GTP gamma S and PMA suggests that the modulatory effect is at the level of protein kinase C.
本研究利用电通透细胞系统来确定Mg.ATP对由次优浓度的fMLP、GTPγS和PMA刺激的中性粒细胞超氧化物（O2-）生成反应的影响。在存在外源添加的Mg.ATP的情况下进行通透处理，既不足以引发O2-释放，对于刺激的O2-产生也不是必需的。然而，在通透处理介质中加入Mg.ATP可引发由这些刺激的次优浓度介导的O2（-）生成反应。Mg.ATP的作用位点在细胞内。此外，Mg.ATP引发由fMLP、GTPγS和PMA刺激的反应这一事实表明，调节作用发生在蛋白激酶C水平。
