Ahmed-Ansari A, Powell J D, Jensen P E, Yehuda-Cohen T, McClure H M, Anderson D, Fultz P N, Sell K W
Department of Pathology and Laboratory Medicine, Winship Cancer Center, Emory University School of Medicine, Atlanta, Georgia 30322.
AIDS. 1990 May;4(5):399-407. doi: 10.1097/00002030-199005000-00004.
The measurement of cell-mediated immunity against the etiologic agent of human AIDS (HIV) in the non-human primate model of AIDS (simian immunodeficiency virus, SIV) has been difficult. In general, culture of peripheral blood mononuclear cells from HIV-1- and SIV-infected humans and monkeys, respectively, with purified inactivated HIV and SIV virus preparations has given inconsistent or negative proliferative responses. However, we describe herein an assay which consists of coculturing monocytes that have been pulsed with inactivated SIVsmm with nylon-wool-purified autologous T cells, leading to antigen-specific T-cell proliferation. The proliferative response, which predominantly occurs in CD4+ T cells, is major histocompatibility complex (MHC) class II-restricted and requires antigen processing. This assay will greatly facilitate the identification of the immunodominant epitopes recognized by T cells in sooty mangabeys, which are naturally infected but remain clinically asymptomatic, and in rhesus macaques, in which experimental infection leads to clinical symptomatology similar to human AIDS, eventually resulting in death.
在艾滋病的非人灵长类动物模型（猴免疫缺陷病毒，SIV）中，针对人类艾滋病病原体（HIV）的细胞介导免疫的测量一直很困难。一般来说，分别用纯化的灭活HIV和SIV病毒制剂培养来自HIV-1和SIV感染的人类和猴子的外周血单核细胞，其增殖反应不一致或呈阴性。然而，我们在此描述了一种检测方法，该方法包括将用灭活的SIVsmm脉冲处理过的单核细胞与尼龙毛纯化的自体T细胞共培养，从而导致抗原特异性T细胞增殖。这种增殖反应主要发生在CD4+ T细胞中，受主要组织相容性复合体（MHC）II类限制，并且需要抗原加工。该检测方法将极大地促进对自然感染但仍无临床症状的乌黑白眉猴以及实验性感染导致与人类艾滋病相似的临床症状并最终导致死亡的恒河猴中T细胞识别的免疫显性表位的鉴定。
