Salama A H, Eisenmann D R, Zaki A E
Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University, Egypt.
Cell Tissue Res. 1990 May;260(3):565-73. doi: 10.1007/BF00297237.
The lysosomal systems in maturation-ameloblasts affected by colchicine were examined using trimetaphosphatase cytochemistry. Demineralized segments of rat incisor were incubated for trimetaphosphatase. At all time intervals, lysosomal structures exhibited reduced enzyme reactivity and were clustered in the Golgi region of the cell. Both ruffle-ended and smooth-ended ameloblasts maintained essentially normal morphology up to 4 h after colchicine injection, except for some migration of organelles. After 8 h, the ruffled border was markedly modified and the associated dense granular material was no longer present. Changes in the lysosomal system and ruffled border indicate interference by colchicine with a putative resorptive function of the maturation-ameloblasts.
利用三磷酸酶细胞化学方法对受秋水仙碱影响的成熟成釉细胞中的溶酶体系统进行了研究。将大鼠切牙的脱矿节段进行三磷酸酶孵育。在所有时间间隔，溶酶体结构的酶活性均降低，并聚集在细胞的高尔基体区域。在注射秋水仙碱后长达4小时，有褶缘和成釉细胞和平滑缘成釉细胞的形态基本保持正常，只是细胞器有一些迁移。8小时后，有褶缘明显改变，相关的致密颗粒物质不再存在。溶酶体系统和有褶缘的变化表明秋水仙碱干扰了成熟成釉细胞的假定吸收功能。
