Department of Physics, University of Warwick, Coventry, CV4 7AL, UK.
Langmuir. 2011 Jul 5;27(13):8269-74. doi: 10.1021/la200639b. Epub 2011 Jun 8.
The force required to form a nanoscale tube from a supported lipid bilayer (SLB) by pulling was measured using an atomic force microscope (AFM). The equilibrium membrane shape during an AFM pulling experiment was calculated and used to derive a general force-distance relationship for pulling a tube from an SLB. We compare these theoretical results with our experimental data and determine the tube radius, the force required to elongate the tube, and, consequently, the surface tension. For a dioleoylphosphatidylcholine (DOPC) SLB, the tension was found to be close to membrane rupture during the pulling experiment.
使用原子力显微镜（AFM）测量了通过拉伸从支撑脂质双层（SLB）形成纳米级管所需的力。在 AFM 拉伸实验中计算了平衡膜形状，并用于推导出从 SLB 中拉伸管的通用力-距离关系。我们将这些理论结果与实验数据进行比较，并确定管半径、拉伸管所需的力，以及由此确定的表面张力。对于二油酰基磷脂酰胆碱（DOPC）SLB，在拉伸实验过程中发现张力接近膜破裂。
