Hsiao C L, Woessner K J, Cheng S M, Dheer S K, Vince T, Lee S G, Hung P P
Biotechnology and Microbiology Division, Wyeth-Ayerst Research, Radnor, PA 19087.
Gene. 1990 May 14;89(2):275-7. doi: 10.1016/0378-1119(90)90017-l.
We report here the cloning and sequencing of the major late promoter (MLP) and the tripartite leader (TPL) from simian adenovirus type 30 (sAd30) and the comparison of the sAd30 nucleotide (nt) sequence with that of human adenoviruses (hAd). The nt sequence homology between sAd30 and hAd2 is 75% from -66 to +190 relative to the cap site. This sAd30 MLP segment contains the upstream regulatory sequence element, TATA box, and downstream regulatory sequence elements that are homologous to hAd MLP. The sAd30 upstream regulatory sequence has a small palindromic DNA sequence GTCACGTGAC, and the TATA box contains the sequence of ATAAA instead of TATAAA. The sAd30 TPL was located on the sAd30 genome as identified by sequence homology with the hAd counterpart. The splice sites of TPL introns were confirmed by sequence analysis of cDNAs synthesized from sAd30-infected cells. There is a 74.2% nt sequence homology between the TPL of sAd30 and hAd2. The conservation of these sequence elements during evolution of Ad suggests that they are essential for the transcription and translation of Ad ML transcripts.
我们在此报告了猿猴腺病毒30型（sAd30）主要晚期启动子（MLP）和三联前导序列（TPL）的克隆与测序，并将sAd30核苷酸（nt）序列与人腺病毒（hAd）的序列进行了比较。相对于帽位点，sAd30与hAd2之间的nt序列同源性在-66至+190之间为75%。该sAd30 MLP片段包含与hAd MLP同源的上游调控序列元件、TATA盒和下游调控序列元件。sAd30上游调控序列有一个小的回文DNA序列GTCACGTGAC，且TATA盒包含的序列为ATAAA而非TATAAA。通过与hAd对应序列的序列同源性鉴定，确定sAd30 TPL位于sAd30基因组上。通过对sAd30感染细胞合成的cDNA进行序列分析，证实了TPL内含子的剪接位点。sAd30与hAd2的TPL之间存在74.2%的nt序列同源性。这些序列元件在腺病毒进化过程中的保守性表明它们对于腺病毒ML转录本的转录和翻译至关重要。
