Department of Experimental and Clinical Physiology, Warsaw Medical University, 00-927 Warsaw, Poland.
Neurosci Lett. 2011 Jul 20;499(2):119-23. doi: 10.1016/j.neulet.2011.05.049. Epub 2011 May 27.
Vasopressin V1a receptors (V1aR) were found in the cerebellum but their functional role has not been determined. As V1aR are engaged in the central regulation of the cardiovascular system and anxiogenic behavior and their role increases in the heart failure and stress, we decided to find out whether expression of V1aR is altered after myocardial infarction and chronic stressing. RT-PCR and Western blot analysis were performed to determine V1aR mRNA and protein expression in the cerebellum of four groups of rats (control sham-operated, infarcted, chronically stressed and infarcted chronically stressed). The myocardial infarct was produced by left coronary artery ligation, and chronic stressing by exposing the rat for four weeks to different types of mild stressors. The rats were sacrificed four weeks after the myocardial surgery or sham operation. Expressions of V1aR mRNA and protein were significantly lower in the infarcted and infarcted chronically stressed rats than in the sham-operated controls and chronically stressed not infarcted rats. No significant differences were found between the sham-operated controls and chronically stressed rats and between the infarcted rats and infarcted rats exposed to chronic stressing. It is concluded that V1aR mRNA and protein expressions are significantly down-regulated in the rats with the post-infarct heart failure but they are not affected by mild chronic stressing.
血管加压素 V1a 受体 (V1aR) 存在于小脑，但它们的功能作用尚未确定。由于 V1aR 参与心血管系统和焦虑行为的中枢调节，并且它们的作用在心力衰竭和应激中增加，我们决定研究心肌梗死后和慢性应激是否会改变 V1aR 的表达。通过 RT-PCR 和 Western blot 分析来确定四组大鼠（对照假手术组、梗死组、慢性应激组和梗死慢性应激组）小脑中的 V1aR mRNA 和蛋白表达。通过左冠状动脉结扎产生心肌梗死，通过将大鼠暴露于四周不同类型的轻度应激源来产生慢性应激。心肌手术后或假手术后四周处死大鼠。与假手术对照组和慢性应激但未梗死的大鼠相比，梗死和梗死慢性应激的大鼠中 V1aR mRNA 和蛋白的表达显著降低。假手术对照组和慢性应激的大鼠之间以及梗死的大鼠和暴露于慢性应激的梗死的大鼠之间没有发现显著差异。结论是，心肌梗死后心力衰竭大鼠的 V1aR mRNA 和蛋白表达显著下调，但轻度慢性应激不影响其表达。
