正电荷 CdTe 量子点的合成及其对尿酸的检测。

Synthesis of positively charged CdTe quantum dots and detection for uric acid.

机构信息

College of Material Science and Engineering, The Key Laboratory of Functional Materials for Fujian Higher Education, Huaqiao University, Xiamen 361021, PR China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2011 Sep;79(5):1566-72. doi: 10.1016/j.saa.2011.05.014. Epub 2011 May 19.

DOI:10.1016/j.saa.2011.05.014

PMID:21652260

Abstract

The CdTe dots (QDs) coated with 2-Mercaptoethylamine was prepared in aqueous solution and characterized with fluorescence spectroscopy, UV-Vis absorption spectra, high-resolution transmission electron microscopy and infrared spectroscopy. When the λex=350 nm, the fluorescence peak of positively charged CdTe quantum dots is at 592 nm. The uric acid is able to quench their fluorescence. Under optimum conditions, the change of fluorescence intensity is linearly proportional to the concentration of uric acid in the range 0.4000-3.600 μmol L(-1), and the limit of detection calculated according to IUPAC definitions is 0.1030 μmol L(-1). Compared with routine method, the present method determines uric acid in human serum with satisfactory results. The mechanism of this strategy is due to the interaction of the tautomeric keto/hydroxyl group of uric acid and the amino group coated at the CdTe QDs.

摘要

巯基乙胺（2-Mercaptoethylamine）修饰的碲化镉量子点（CdTe dots）在水溶液中合成，并通过荧光光谱、紫外-可见吸收光谱、高分辨率透射电子显微镜和红外光谱进行了表征。当 λex=350nm 时，正电荷 CdTe 量子点的荧光峰位于 592nm。尿酸能够猝灭其荧光。在最佳条件下，荧光强度的变化与尿酸浓度在 0.4000-3.600μmol L(-1)范围内呈线性关系，根据 IUPAC 定义计算的检出限为 0.1030μmol L(-1)。与常规方法相比，本方法用于测定人血清中的尿酸，结果令人满意。该策略的机制归因于尿酸的互变异构酮/羟基与 CdTe QDs 表面包裹的氨基之间的相互作用。