Zhao Wenfeng, Fung Yingsing, O Waisum, Cheung M P L
Department of Chemistry, The University of Hong Kong, Hong Kong SAR, PR China.
Anal Sci. 2010;26(8):879-84. doi: 10.2116/analsci.26.879.
This paper described the investigation of surface-modified quantum dots (QDs) as a fluorescence probe for the detection of cardiolipin. A single-step method for preparation of non-toxic and photo-stable cadmium telluride (CdTe) QDs capped by L-cysteine in aqueous solution was developed. The prepared QDs were characterized by high-resolution transmission electron microscopy, X-ray diffraction spectrometry, Fourier transform infrared spectrometry and spectrofluorometry. These functional QDs were used as a fluorescence probe for cardiolipin determination based on the fluorescence quenching. The optimum fluorescence intensity was found to be at pH 7.4 with QDs concentration of 4 x 10(-5) mol L(-1). The effect of other phospholipids on the intensity of CdTe QDs showed a low interference response. Under optimized conditions, the quenched fluorescence intensity was linear with the concentration of cardiolipin in the range of 1.33 x 10(-7) - 10.4 x 10(-7) mol L(-1) (r = 0.9976) and a detection limit (S/N = 3) of 18.5 nmol L(-1). The proposed method was applied to the determination of cardiolipin content of HepG2 cell samples before and after oxidative stress with satisfactory results.
本文描述了对表面修饰量子点（QDs）作为检测心磷脂的荧光探针的研究。开发了一种在水溶液中制备由L-半胱氨酸包覆的无毒且光稳定的碲化镉（CdTe）量子点的单步方法。通过高分辨率透射电子显微镜、X射线衍射光谱、傅里叶变换红外光谱和荧光光谱对制备的量子点进行了表征。这些功能性量子点基于荧光猝灭用作心磷脂测定的荧光探针。发现最佳荧光强度在pH 7.4且量子点浓度为4×10⁻⁵ mol L⁻¹时。其他磷脂对CdTe量子点强度的影响显示出低干扰响应。在优化条件下，猝灭的荧光强度与心磷脂浓度在1.33×10⁻⁷ - 10.4×10⁻⁷ mol L⁻¹范围内呈线性关系（r = 0.9976），检测限（S/N = 3）为18.5 nmol L⁻¹。所提出的方法应用于氧化应激前后HepG2细胞样品中心磷脂含量的测定，结果令人满意。
Zotero 插件