Department of Hematology, University of Copenhagen, 2200 Copenhagen-N, Denmark.
J Leukoc Biol. 2011 Sep;90(3):629-34. doi: 10.1189/jlb.0311123. Epub 2011 Jun 7.
The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.
本研究报告了一种基于流式细胞术的方案，用于前瞻性纯化代表终末中性粒细胞分化的六个连续阶段的人 BM 群体，包括早期前髓细胞和晚期前髓细胞、髓细胞、成髓细胞、带细胞和 PMN 中性粒细胞。验证实验显示，每种骨髓群体的纯度都很高，并且在前所未有的分辨率下表达了中性粒细胞分化的标记基因的有生物学意义的表达谱。因此，本方案应该有助于研究人类体内中性粒细胞的分化，并构成了基于体外髓系细胞系和 HPC 分化模型的重要替代方案。
