枯草芽孢杆菌天冬氨酸激酶I对中-二氨基庚二酸别构抑制的脱敏作用使天冬氨酸激酶I在指数生长期的氨基酸生物合成中发挥作用。
Desensitization of Bacillus subtilis aspartokinase I to allosteric inhibition by meso-diaminopimelate allows aspartokinase I to function in amino acid biosynthesis during exponential growth.
作者信息
Zhang J J, Paulus H
机构信息
Department of Metabolic Regulation, Boston Biomedical Research Institute, Massachusetts 02114.
出版信息
J Bacteriol. 1990 Aug;172(8):4690-3. doi: 10.1128/jb.172.8.4690-4693.1990.
Abstract
Strains of Bacillus subtilis deficient in aspartokinases II and III are unable to grow in the absence of lysine, methionine, and threonine, although they have normal levels of aspartokinase I (J.J. Zhang, F.M. Hu, N.Y. Chen, and H. Paulus, J. Bacteriol. 172:701-708, 1990). Revertants with the ability to grow in the absence of lysine and methionine had an altered aspartokinase I, which was insensitive to feedback inhibition by meso-diaminopimelate. This suggests that inhibition by meso-diaminopimelate limits the ability of aspartokinase I to function in amino acid biosynthesis.
摘要
枯草芽孢杆菌中缺乏天冬氨酸激酶II和III的菌株，即便天冬氨酸激酶I水平正常，在没有赖氨酸、蛋氨酸和苏氨酸的情况下也无法生长（J.J. Zhang、F.M. Hu、N.Y. Chen和H. Paulus，《细菌学杂志》172:701 - 708，1990年）。具有在没有赖氨酸和蛋氨酸的情况下生长能力的回复突变体，其天冬氨酸激酶I发生了改变，对中-二氨基庚二酸的反馈抑制不敏感。这表明中-二氨基庚二酸的抑制作用限制了天冬氨酸激酶I在氨基酸生物合成中发挥作用的能力。
相似文献
1
Desensitization of Bacillus subtilis aspartokinase I to allosteric inhibition by meso-diaminopimelate allows aspartokinase I to function in amino acid biosynthesis during exponential growth.枯草芽孢杆菌天冬氨酸激酶I对中-二氨基庚二酸别构抑制的脱敏作用使天冬氨酸激酶I在指数生长期的氨基酸生物合成中发挥作用。
J Bacteriol. 1990 Aug;172(8):4690-3. doi: 10.1128/jb.172.8.4690-4693.1990.
2
Comparison of the three aspartokinase isozymes in Bacillus subtilis Marburg and 168.枯草芽孢杆菌马尔堡菌株和168菌株中三种天冬氨酸激酶同工酶的比较。
J Bacteriol. 1990 Feb;172(2):701-8. doi: 10.1128/jb.172.2.701-708.1990.
3
Expression of the gene for Bacillus subtilis aspartokinase II in Escherichia coli.枯草芽孢杆菌天冬氨酸激酶II基因在大肠杆菌中的表达。
J Biol Chem. 1985 Jan 10;260(1):592-7.
4
Activities and regulation of the enzymes involved in the first and the third steps of the aspartate biosynthetic pathway in Enterococcus faecium.粪肠球菌中天冬氨酸生物合成途径第一步和第三步所涉及酶的活性及调控
Arch Microbiol. 1994;161(4):359-62. doi: 10.1007/BF00303593.
5
Aspartokinase III, a new isozyme in Bacillus subtilis 168.天冬氨酸激酶III，枯草芽孢杆菌168中的一种新的同工酶。
J Bacteriol. 1990 Jan;172(1):218-23. doi: 10.1128/jb.172.1.218-223.1990.
6
Lysine- and lysine-plus-threonine-inhibitable aspartokinases in Bacillus brevis.短短芽孢杆菌中赖氨酸及赖氨酸加苏氨酸可抑制的天冬氨酸激酶
Biochim Biophys Acta. 1976 Sep 14;445(2):350-63. doi: 10.1016/0005-2744(76)90089-9.
7
Genes involved in meso-diaminopimelate synthesis in Bacillus subtilis: identification of the gene encoding aspartokinase I.
J Gen Microbiol. 1991 Apr;137(4):951-62. doi: 10.1099/00221287-137-4-951.
8
Aspartokinase isoenzymes of the fruiting myxobacterium Myxoccus xanthus.黏细菌黄单胞菌产孢期的天冬氨酸激酶同工酶。
Biochim Biophys Acta. 1975 Oct 22;403(2):345-54. doi: 10.1016/0005-2744(75)90064-9.
9
10
Regulation of two aspartokinase isozymes in Streptococcus bovis.牛链球菌中两种天冬氨酸激酶同工酶的调控
Can J Microbiol. 1994 Mar;40(3):224-7. doi: 10.1139/m94-036.
引用本文的文献
1
Analysis and manipulation of aspartate pathway genes for L-lysine overproduction from methanol by Bacillus methanolicus.分析和操作天冬氨酸途径基因，以提高甲醇嗜甲基芽孢杆菌生产 L-赖氨酸的产量。
Appl Environ Microbiol. 2011 Sep;77(17):6020-6. doi: 10.1128/AEM.05093-11. Epub 2011 Jul 1.
2
Cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.凝聚群方法在天冬氨酸激酶进化分析中的应用，天冬氨酸激酶是一种为许多生化途径的分支网络提供养分的酶。
Microbiol Mol Biol Rev. 2009 Dec;73(4):594-651. doi: 10.1128/MMBR.00024-09.
3
Overexpression of wild-type aspartokinase increases L-lysine production in the thermotolerant methylotrophic bacterium Bacillus methanolicus.野生型天冬氨酸激酶的过表达增加了嗜热甲基营养型细菌甲醇芽孢杆菌中L-赖氨酸的产量。
Appl Environ Microbiol. 2009 Feb;75(3):652-61. doi: 10.1128/AEM.01176-08. Epub 2008 Dec 5.
4
Cloning and nucleotide sequence of the gene coding for aspartokinase II from a thermophilic methylotrophic Bacillus sp.嗜热甲基营养芽孢杆菌中天冬氨酸激酶II编码基因的克隆与核苷酸序列分析
Appl Environ Microbiol. 1992 Sep;58(9):2806-14. doi: 10.1128/aem.58.9.2806-2814.1992.
本文引用的文献
1
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌转化的要求。
J Bacteriol. 1961 May;81(5):741-6. doi: 10.1128/jb.81.5.741-746.1961.
2
Regulation of aspartokinase in Bacillus subtilis. The separation and properties of two isofunctional enzymes.枯草芽孢杆菌中天冬氨酸激酶的调控。两种同功酶的分离与特性
J Biol Chem. 1971 May 10;246(9):2965-71.
3
Biosynthesis of dipicolinic acid in Bacillus subtilis.
Biochem Biophys Res Commun. 1967 Dec 15;29(5):648-54. doi: 10.1016/0006-291x(67)90265-3.
4
Cloning and structure of the gene for the subunits of aspartokinase II from Bacillus subtilis.枯草芽孢杆菌天冬氨酸激酶II亚基基因的克隆与结构
J Biol Chem. 1985 Jan 10;260(1):585-91.
5
Comparison of the three aspartokinase isozymes in Bacillus subtilis Marburg and 168.枯草芽孢杆菌马尔堡菌株和168菌株中三种天冬氨酸激酶同工酶的比较。
J Bacteriol. 1990 Feb;172(2):701-8. doi: 10.1128/jb.172.2.701-708.1990.
6
Aspartokinase III, a new isozyme in Bacillus subtilis 168.天冬氨酸激酶III，枯草芽孢杆菌168中的一种新的同工酶。
J Bacteriol. 1990 Jan;172(1):218-23. doi: 10.1128/jb.172.1.218-223.1990.
7
Control of lysine biosynthesis in Bacillus subtilis: inhibition of diaminopimelate decarboxylase by lysine.枯草芽孢杆菌中赖氨酸生物合成的调控：赖氨酸对二氨基庚二酸脱羧酶的抑制作用。
J Bacteriol. 1975 Jan;121(1):20-8. doi: 10.1128/jb.121.1.20-28.1975.
Zotero 插件