Ryerse J S, Beck J R, Lavrik P B
Department of Pathology, St. Louis University Medical School, Missouri 63104.
J Invertebr Pathol. 1990 Jul;56(1):86-90. doi: 10.1016/0022-2011(90)90148-y.
Light microscope immunofluorescence was used to localize the membrane binding of Bacillus thuringiensis kurstaki 63-kDa delta-endotoxin in Heliothis virescens midgut and Malpighian tubules. Staining was observed along all exposed mucosal (apical microvillar) plasma membranes. Interpretation of the serosal (basal) plasma membrane staining was complicated because the basal lamina also stained. The results suggest that the toxin binds to all exposed plasma membranes without apparent specificity for particular membrane domains.
利用光学显微镜免疫荧光技术对苏云金芽孢杆菌库尔斯塔克亚种63-kDaδ-内毒素在烟草天蛾中肠和马氏管中的膜结合情况进行定位。在所有暴露的黏膜（顶端微绒毛）质膜上均观察到染色。由于基膜也有染色，所以对浆膜（基底）质膜染色的解读较为复杂。结果表明，该毒素可结合到所有暴露的质膜上，且对特定膜结构域无明显特异性。
