Ts'o D Y, Frostig R D, Lieke E E, Grinvald A
Laboratory of Neurobiology, Rockefeller University, New York, NY 10021.
Science. 1990 Jul 27;249(4967):417-20. doi: 10.1126/science.2165630.
A high spatial resolution optical imaging system was developed to visualize cerebral cortical activity in vivo. This method is based on activity-dependent intrinsic signals and does not use voltage-sensitive dyes. Images of the living monkey striate (VI) and extrastriate (V2) visual cortex, taken during visual stimulation, were analyzed to yield maps of the distribution of cells with various functional properties. The cytochrome oxidase--rich blobs of V1 and the stripes of V2 were imaged in the living brain. In V2, no ocular dominance organization was seen, while regions of poor orientation tuning colocalized to every other cytochrome oxidase stripe. The orientation tuning of other regions of V2 appeared organized as modules that are larger and more uniform than those in V1.
开发了一种高空间分辨率光学成像系统，用于在体内可视化大脑皮层活动。该方法基于活动依赖的内在信号，不使用电压敏感染料。分析了在视觉刺激期间拍摄的活猴纹状（VI）和纹外（V2）视觉皮层的图像，以生成具有各种功能特性的细胞分布图。在活体大脑中对V1富含细胞色素氧化酶的斑点和V2的条纹进行了成像。在V2中，未观察到眼优势组织，而定向调谐较差的区域与每隔一条细胞色素氧化酶条纹共定位。V2其他区域的定向调谐似乎组织成比V1中更大且更均匀的模块。
