Gulliksson H
Stockholm Blood Transfusion Service, Sweden.
Transfusion. 1990 Jul-Aug;30(6):536-40. doi: 10.1046/j.1537-2995.1990.30690333486.x.
The level of extracellular lactate dehydrogenase (LDH) activity is often used as an indicator for the leakage of cytosolic platelet contents. The objective of this investigation was to study the possibility of using a different enzyme, adenylate kinase (AK), as a substitute for LDH. A bioluminescent assay was used, and three different platelet storage containers were studied. In a comparative study of AK and LDH activities during platelet storage, similar results were obtained, but some differences were also observed. Because more AK (in percentages) than LDH is contained in platelets, the initial levels of AK are lower than those of LDH. The release of AK activity from damaged platelets is delayed or is somewhat lower than that of LDH. The amount of AK activity released per platelet is correlated with the volume of the platelet units. A smaller volume entails increased AK activity. This observation applies to all storage conditions investigated, and the results in one of the containers indicate that this is also true for LDH. The results of the investigation demonstrate that similar information is obtained with LDH and AK. AK can be used as an alternative to LDH for studies of lytic processes during platelet storage.
细胞外乳酸脱氢酶（LDH）活性水平常被用作胞质血小板内容物泄漏的指标。本研究的目的是探讨使用另一种酶——腺苷酸激酶（AK）替代LDH的可能性。采用了生物发光测定法，并研究了三种不同的血小板储存容器。在血小板储存过程中对AK和LDH活性进行的比较研究中，获得了相似的结果，但也观察到了一些差异。由于血小板中所含的AK（以百分比计）比LDH多，所以AK的初始水平低于LDH。受损血小板释放AK活性的过程会延迟，或者比释放LDH的过程略低。每个血小板释放的AK活性量与血小板单位的体积相关。体积越小，AK活性越高。这一观察结果适用于所有研究的储存条件，其中一个容器的结果表明LDH也是如此。研究结果表明，使用LDH和AK可获得相似的信息。在研究血小板储存过程中的溶解过程时，AK可作为LDH的替代物。
