TCM Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Phytother Res. 2012 Feb;26(2):231-8. doi: 10.1002/ptr.3492. Epub 2011 Jun 7.
The study investigated the beneficial effects of astragalosides (AS) on cardiac performance in rats with chronic heart failure. Chronic heart failure was produced by left anterior descending coronary artery ligation, and the therapeutic efficacy of astragalosides at 10, 20 and 40 mg/kg was evaluated. Five weeks after the operation, cardiac function was deficient and sarcoplasmic reticulum Ca²⁺-ATPase (SERCA) activity was significantly reduced. Moreover, SERCA mRNA decreased, while expression of the SERCA down-regulator phospholamban (PLB) was significantly increased. Phosphorylated phospholamban (P-PLB), the form that does not inhibit SERCA, was also reduced by chronic heart failure. Treatment with AS improved left ventricle function and cardiac structure, reversed the depression of SERCA activity, and increased P-PLB. These results suggest that the cardioprotective effect of AS may be due to the increase in P-PLB protein, which disinhibits SERCA activity. Rescue of sarcoplasmic reticulum Ca²⁺ cycling by astragalosides could normalize excitation-contraction coupling and improve overall cardiac function.
该研究探讨了黄芪甲苷（AS）对慢性心力衰竭大鼠心功能的有益作用。通过结扎左前降支冠状动脉造成慢性心力衰竭，并评估黄芪甲苷在 10、20 和 40mg/kg 时的治疗效果。手术后 5 周，心功能不足，肌浆网 Ca²⁺-ATP 酶（SERCA）活性显著降低。此外，SERCA mRNA 减少，而 SERCA 下调调节剂磷酸化肌球蛋白轻链 2（PLB）的表达显著增加。慢性心力衰竭还使不抑制 SERCA 的磷酸化 PLB（P-PLB）形式减少。AS 治疗可改善左心室功能和心脏结构，逆转 SERCA 活性的抑制，并增加 P-PLB。这些结果表明，AS 的心脏保护作用可能是由于 P-PLB 蛋白的增加，从而解除了 SERCA 的抑制。黄芪甲苷对肌浆网 Ca²⁺循环的挽救可以使兴奋-收缩偶联正常化，并改善整体心脏功能。
