Medina J F, Barrios C, Funk C D, Larsson O, Haeggström J, Rådmark O
Department of Physiological Chemistry, karolinska Institutet, Stockholm, Sweden.
Eur J Biochem. 1990 Jul 20;191(1):27-31. doi: 10.1111/j.1432-1033.1990.tb19089.x.
Human fibroblasts in cell culture converted the epoxide intermediate leukotriene A4 into the potent chemotaxin leukotriene B4. The identity of leukotriene B4 was ascertained by its mobility in reverse-phase high performance liquid chromatography, ultraviolet spectroscopy and gas chromatography/mass spectrometry. The presence of the enzyme responsible for the conversion (i.e. leukotriene A4 hydrolase), as well as the corresponding mRNA, were demonstrated by Western and Northern blot analyses. Leukotriene-A4-hydrolase enzyme activity, protein and mRNA were all enhanced (approximately threefold) in human fibroblasts that had been transformed by simian virus 40.
