Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, Portugal.
Plant Signal Behav. 2011 Jun;6(6):895-7. doi: 10.4161/psb.6.6.15321. Epub 2011 Jun 1.
Cardosins are abundant in cardoon pistils and were found to accumulate in different cell compartments: cardosin A was detected in the vacuoles of stigmatic papillae and cardosin B accumulates in the extracellular matrix of the transmitting tissue. Due to the fact that cardoon system imposes some limitations to the study of processing and trafficking events, heterologous species have been employed to study cardosins trafficking pathways. Cardosin B was successfully expressed both in Arabidopsis and Tobacco plants, where it accumulated mainly in the vacuole but it was also detected in the cell wall. The glycosylation pattern of cardosin B was replicated in these systems - high-mannose type glycans. In tobacco leaves, cardosin B is transported through the Golgi in a RAB-D2a-dependent route, and is delivered to the vacuole via the prevacuolar compartment in a RAB-F2b-dependent pathway.
蓟心蛋白在蓟的雌蕊中含量丰富，并且被发现积累在不同的细胞隔室中：在柱头乳突的液泡中检测到蓟心蛋白 A，而蓟心蛋白 B 则积聚在传递组织的细胞外基质中。由于蓟系统对加工和运输事件的研究施加了一些限制，因此已经采用了异源物种来研究蓟心蛋白的运输途径。蓟心蛋白 B 在拟南芥和烟草植物中均成功表达，主要在液泡中积累，但也在细胞壁中检测到。这些系统中复制了蓟心蛋白 B 的糖基化模式——高甘露糖型聚糖。在烟草叶片中，蓟心蛋白 B 通过 RAB-D2a 依赖性途径在高尔基体中运输，并通过 RAB-F2b 依赖性途径通过前液泡区室递送至液泡。
