用于链间交联修复的同源重组检测
Homologous recombination assay for interstrand cross-link repair.
作者信息
Nakanishi Koji, Cavallo Francesca, Brunet Erika, Jasin Maria
机构信息
Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
出版信息
Methods Mol Biol. 2011;745:283-91. doi: 10.1007/978-1-61779-129-1_16.
Abstract
DNA interstrand cross-links (ICLs) covalently link both strands of the DNA duplex, impeding cellular processes like DNA replication. Homologous recombination (HR) is considered to be a major pathway for the repair of ICLs in mammalian cells as mutants for HR components are highly sensitive to DNA-damaging agents that cause ICLs. This chapter describes GFP assays to measure HR following site-specific ICL formation with psoralen through DNA triplex technology. This approach can be used to determine the genetic requirements for ICL-induced HR in relation to those involved in HR repair of other DNA lesions such as double-strand breaks.
摘要
DNA链间交联（ICL）共价连接DNA双链的两条链，阻碍DNA复制等细胞过程。同源重组（HR）被认为是哺乳动物细胞中ICL修复的主要途径，因为HR组分的突变体对导致ICL的DNA损伤剂高度敏感。本章描述了通过DNA三链体技术用补骨脂素进行位点特异性ICL形成后测量HR的绿色荧光蛋白（GFP）检测方法。这种方法可用于确定ICL诱导的HR相对于参与其他DNA损伤（如双链断裂）的HR修复的遗传需求。
相似文献
1
Homologous recombination assay for interstrand cross-link repair.用于链间交联修复的同源重组检测
Methods Mol Biol. 2011;745:283-91. doi: 10.1007/978-1-61779-129-1_16.
2
Assessing Homologous Recombination and Interstrand Cross-Link Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines.评估胚胎性癌睾丸生殖细胞肿瘤细胞系中的同源重组和链间交联修复。
Methods Mol Biol. 2021;2195:113-123. doi: 10.1007/978-1-0716-0860-9_9.
3
Involvement of nucleotide excision repair in a recombination-independent and error-prone pathway of DNA interstrand cross-link repair.核苷酸切除修复参与DNA链间交联修复的一种不依赖重组且易出错的途径。
Mol Cell Biol. 2001 Feb;21(3):713-20. doi: 10.1128/MCB.21.3.713-720.2001.
4
Double-strand breaks induce homologous recombinational repair of interstrand cross-links via cooperation of MSH2, ERCC1-XPF, REV3, and the Fanconi anemia pathway.双链断裂通过MSH2、ERCC1-XPF、REV3和范可尼贫血途径的协同作用诱导链间交联的同源重组修复。
DNA Repair (Amst). 2007 Nov;6(11):1670-8. doi: 10.1016/j.dnarep.2007.06.002. Epub 2007 Jul 31.
5
Nucleotide excision repair- and polymerase eta-mediated error-prone removal of mitomycin C interstrand cross-links.核苷酸切除修复和聚合酶η介导的丝裂霉素C链间交联的易错去除。
Mol Cell Biol. 2003 Jan;23(2):754-61. doi: 10.1128/MCB.23.2.754-761.2003.
6
DNA interstrand crosslinks induce a potent replication block followed by formation and repair of double strand breaks in intact mammalian cells.DNA 链间交联在完整的哺乳动物细胞中诱导强烈的复制阻滞，随后形成和修复双链断裂。
DNA Repair (Amst). 2012 Dec 1;11(12):976-85. doi: 10.1016/j.dnarep.2012.09.010. Epub 2012 Oct 23.
7
Repair of DNA interstrand cross-links: interactions between homology-dependent and homology-independent pathways.DNA链间交联的修复：同源依赖性途径与非同源依赖性途径之间的相互作用
DNA Repair (Amst). 2006 May 10;5(5):566-74. doi: 10.1016/j.dnarep.2006.01.010. Epub 2006 Mar 29.
8
Advances in understanding the complex mechanisms of DNA interstrand cross-link repair.深入了解 DNA 链间交联修复复杂机制的进展。
Cold Spring Harb Perspect Biol. 2013 Oct 1;5(10):a012732. doi: 10.1101/cshperspect.a012732.
9
Targeting and processing of site-specific DNA interstrand crosslinks.靶向和处理特定部位 DNA 链间交联。
Environ Mol Mutagen. 2010 Jul;51(6):527-39. doi: 10.1002/em.20557.
10
Defining the roles of nucleotide excision repair and recombination in the repair of DNA interstrand cross-links in mammalian cells.确定核苷酸切除修复和重组在哺乳动物细胞DNA链间交联修复中的作用。
Mol Cell Biol. 2000 Nov;20(21):7980-90. doi: 10.1128/MCB.20.21.7980-7990.2000.
引用本文的文献
1
Cyclin D1 overexpression induces replication stress and microhomology-mediated end-joining dependence in mantle cell lymphoma.细胞周期蛋白D1过表达在套细胞淋巴瘤中诱导复制应激和微同源性介导的末端连接依赖性。
J Clin Invest. 2025 Jul 3;135(17). doi: 10.1172/JCI193006. eCollection 2025 Sep 2.
2
RAD51 plays critical roles in DNMT1-mediated maintenance methylation of genomic DNA by dually regulating the ubiquitin ligase UHRF1.RAD51通过双重调节泛素连接酶UHRF1，在DNA甲基转移酶1介导的基因组DNA维持甲基化过程中发挥关键作用。
Proc Natl Acad Sci U S A. 2024 Dec 10;121(50):e2410119121. doi: 10.1073/pnas.2410119121. Epub 2024 Dec 2.
3
Onvansertib treatment overcomes olaparib resistance in high-grade ovarian carcinomas.奥凡妥昔单抗治疗克服高级别卵巢癌中奥拉帕利的耐药性。
Cell Death Dis. 2024 Jul 22;15(7):521. doi: 10.1038/s41419-024-06894-1.
4
Increased Synthetic Cytotoxicity of Combinatorial Chemoradiation Therapy in Homologous Recombination Deficient Tumors.同源重组缺陷肿瘤中联合放化疗的合成细胞毒性增加。
Int J Radiat Oncol Biol Phys. 2025 Mar 1;121(3):768-779. doi: 10.1016/j.ijrobp.2024.06.037. Epub 2024 Jul 10.
5
Replication-Independent ICL Repair: From Chemotherapy to Cell Homeostasis.复制独立型 ICL 修复：从化疗到细胞稳态。
J Mol Biol. 2024 Jul 1;436(13):168618. doi: 10.1016/j.jmb.2024.168618. Epub 2024 May 18.
6
ZNF827 is a single-stranded DNA binding protein that regulates the ATR-CHK1 DNA damage response pathway.锌指蛋白827是一种单链DNA结合蛋白，可调节ATR-CHK1 DNA损伤反应通路。
Nat Commun. 2024 Mar 12;15(1):2210. doi: 10.1038/s41467-024-46578-0.
7
The protein phosphatase EYA4 promotes homologous recombination (HR) through dephosphorylation of tyrosine 315 on RAD51.蛋白磷酸酶 EYA4 通过去磷酸化 RAD51 上的酪氨酸 315 促进同源重组 (HR)。
Nucleic Acids Res. 2024 Feb 9;52(3):1173-1187. doi: 10.1093/nar/gkad1177.
8
STAG2 Regulates Homologous Recombination Repair and Sensitivity to ATM Inhibition.STAG2 调控同源重组修复及对 ATM 抑制的敏感性。
Adv Sci (Weinh). 2023 Dec;10(36):e2302494. doi: 10.1002/advs.202302494. Epub 2023 Nov 20.
9
FANCD2 and RAD51 recombinase directly inhibit DNA2 nuclease at stalled replication forks and FANCD2 acts as a novel RAD51 mediator in strand exchange to promote genome stability.FANCD2 和 RAD51 重组酶在停滞的复制叉处直接抑制 DNA2 核酸酶，并且 FANCD2 作为一种新型 RAD51 介体在链交换中起作用，以促进基因组稳定性。
Nucleic Acids Res. 2023 Sep 22;51(17):9144-9165. doi: 10.1093/nar/gkad624.
10
ATR inhibition augments the efficacy of lurbinectedin in small-cell lung cancer.ATR 抑制增强了鲁比卡丁在小细胞肺癌中的疗效。
EMBO Mol Med. 2023 Aug 7;15(8):e17313. doi: 10.15252/emmm.202217313. Epub 2023 Jul 25.
本文引用的文献
1
Homology-directed Fanconi anemia pathway cross-link repair is dependent on DNA replication.同源定向的范可尼贫血通路交联修复依赖于 DNA 复制。
Nat Struct Mol Biol. 2011 Apr;18(4):500-3. doi: 10.1038/nsmb.2029. Epub 2011 Mar 20.
2
Using synthetic DNA interstrand crosslinks to elucidate repair pathways and identify new therapeutic targets for cancer chemotherapy.利用合成 DNA 链间交联物来阐明修复途径，并为癌症化疗确定新的治疗靶点。
Cell Mol Life Sci. 2010 Nov;67(21):3683-97. doi: 10.1007/s00018-010-0492-6. Epub 2010 Aug 21.
3
Role of homologous recombination in DNA interstrand crosslink repair.同源重组在 DNA 链间交联修复中的作用。
Environ Mol Mutagen. 2010 Jul;51(6):582-603. doi: 10.1002/em.20577.
4
A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.全基因组规模的 DNA 修复 RNAi 筛选鉴定出 SPG48 是一种与遗传性痉挛性截瘫相关的新基因。
PLoS Biol. 2010 Jun 29;8(6):e1000408. doi: 10.1371/journal.pbio.1000408.
5
Mitotic homologous recombination maintains genomic stability and suppresses tumorigenesis.有丝分裂同源重组维持基因组稳定性并抑制肿瘤发生。
Nat Rev Mol Cell Biol. 2010 Mar;11(3):196-207. doi: 10.1038/nrm2851.
6
Fanconi anemia and its diagnosis.范可尼贫血及其诊断。
Mutat Res. 2009 Jul 31;668(1-2):4-10. doi: 10.1016/j.mrfmmm.2009.01.013. Epub 2009 Feb 28.
7
Repair of DNA lesions associated with triplex-forming oligonucleotides.与三链形成寡核苷酸相关的DNA损伤修复
Mol Carcinog. 2009 Apr;48(4):389-99. doi: 10.1002/mc.20501.
8
Mechanism of replication-coupled DNA interstrand crosslink repair.复制偶联的DNA链间交联修复机制。
Cell. 2008 Sep 19;134(6):969-80. doi: 10.1016/j.cell.2008.08.030.
9
Emergence of a DNA-damage response network consisting of Fanconi anaemia and BRCA proteins.由范可尼贫血蛋白和乳腺癌易感基因（BRCA）蛋白组成的DNA损伤反应网络的出现。
Nat Rev Genet. 2007 Oct;8(10):735-48. doi: 10.1038/nrg2159. Epub 2007 Sep 4.
10
Targeting chromosomal sites with locked nucleic acid-modified triplex-forming oligonucleotides: study of efficiency dependence on DNA nuclear environment.用锁核酸修饰的三链形成寡核苷酸靶向染色体位点：效率对DNA核环境依赖性的研究
Nucleic Acids Res. 2006;34(16):4546-53. doi: 10.1093/nar/gkl630. Epub 2006 Sep 1.
Zotero 插件