Murray A B, Becke H, Taccagni G L
GSF Institute of Pathology, Neuherberg/München, Federal Republic of Germany.
Ultrastruct Pathol. 1990 Jul-Aug;14(4):335-42. doi: 10.3109/01913129009032248.
A case of extraskeletal osteosarcoma was observed in the thigh of a 33-year-old male patient. Ultrastructurally the tumor was characterized by the presence of a particular dense type of cell, the nucleus of which showed a characteristic combination of features: large amounts of condensed marginated chromatin, prominent perichromatin granules, vermicellar bodies, and undulating microtubules. The tumor also contained intermediate-type cells with a more typical osteoblastic appearance, and more blastic cells. All three cell types contained varying amounts of dilated rough endoplasmic reticulum with prominent inclusions of crystalline material showing a hexagonal or banded pattern, indicating that the cells represent different stages of maturation rather than genuinely different types of cells. Dense cells showing the same characteristic combination of nuclear features have been described once before in a case of parosteal osteosarcoma. Our results indicate that these cells are a particular form of osteogenic cell. The presence of undulating microtubules and vermicellar bodies suggest a possible association with the presence of virus and/or increased levels of interferon.
一名33岁男性患者大腿处发现了1例骨外骨肉瘤。超微结构上，肿瘤的特征是存在一种特殊的致密型细胞，其细胞核呈现出一组特征性组合：大量浓缩的边缘染色质、明显的核周颗粒、蠕虫样小体和波浪状微管。肿瘤还含有具有更典型成骨细胞外观的中间型细胞以及更多的母细胞。所有这三种细胞类型都含有不同数量的扩张型粗面内质网，其中含有突出的呈六边形或带状图案的晶体物质包涵体，这表明这些细胞代表了不同的成熟阶段，而非真正不同类型的细胞。致密细胞呈现出相同的细胞核特征性组合，之前在1例骨旁骨肉瘤病例中曾有过描述。我们的结果表明，这些细胞是成骨细胞的一种特殊形式。波浪状微管和蠕虫样小体的存在提示可能与病毒存在和/或干扰素水平升高有关。
