Hart-Elcock L, Baker R D, Leipold H W
Department of Pathology, College of Veterinary Medicine, Kansas State University, Manhattan.
Zentralbl Veterinarmed A. 1990 May;37(4):294-9. doi: 10.1111/j.1439-0442.1990.tb00908.x.
Testing for bovine hereditary syndactyly used artificial insemination, superovulation, and embryo transfer. Thirty-two suspect bulls and 9 suspect cows were mated with 10 syndactyly cows and two syndactylous bulls, respectively. 209 embryos were recovered and one, two, or three embryos was/were transfered into each recipient cow and 56 to 77 days after transfer, 174 fetuses were recovered. There was a significant relationship between amniotic fluid weights from the left uterine horn, fetal weights from right and left uterine horns, and fetal sex, side of corpus luteum, and number of embryo implants. Fetal membrane weights, amniotic fluid weights, fetal weights, and crown-rump lengths increased significantly with fetal age, but recipient cow weight had no significant effect. Survival data from single, double, and triple embryo implants indicated that it was most profitable under these test conditions to implant two embryos per recipient cow, one in each uterine horn.
牛遗传性并趾畸形的检测采用人工授精、超数排卵和胚胎移植技术。分别让32头疑似公牛和9头疑似母牛与10头并趾畸形母牛和2头并趾畸形公牛交配。共回收209枚胚胎,将1枚、2枚或3枚胚胎分别移植到每头受体母牛体内,移植后56至77天,回收174头胎儿。左子宫角羊水重量、左右子宫角胎儿重量与胎儿性别、黄体侧别及胚胎植入数量之间存在显著关系。胎膜重量、羊水重量、胎儿重量和顶臀长度随胎龄显著增加,但受体母牛体重无显著影响。单胚胎、双胚胎和三胚胎植入的存活数据表明,在这些试验条件下,每头受体母牛在每个子宫角各植入1枚胚胎,共植入2枚胚胎最有利可图。