Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd., Shanghai 200032, China.
Bioresour Technol. 2011 Aug;102(16):7407-14. doi: 10.1016/j.biortech.2011.05.035. Epub 2011 May 20.
An anaerobic baffled reactor with four compartments (C1-C4) was successfully used for treatment of acetone-butanol-ethanol fermentation wastewater and methane production. The chemical oxygen demand (COD) removal efficiency was 88.2% with a CH(4) yield of 0.25L/(g COD(removed)) when organic loading rate (OLR) was 5.4kg CODm(-3)d(-1). C1 played the most important role in solvents (acetone, butanol and ethanol) and COD removal. Community structure of C2 was similar to that in C1 at stage 3 with higher OLR, but was similar to those in C3 and C4 at stages 1-2 with lower OLR. This community variation in C2 was consistent with its increased role in COD and solvent removal at stage 3. During community succession from C1 to C4 at stage 3, abundance of Firmicutes (especially OTUs ABRB07 and ABRB10) and Methanoculleus decreased, while Bacteroidetes and Methanocorpusculum became dominant. Thus, ABRB07 coupled with Methanoculleus and/or acetogen (ABRB10) may be key species for solvents degradation.
采用四隔室（C1-C4）厌氧折流板反应器成功处理丙酮-丁醇-乙醇发酵废水并进行甲烷生产。当有机负荷率（OLR）为 5.4kg CODm(-3)d(-1)时，COD 去除率为 88.2%，CH(4)产率为 0.25L/(g COD(去除))。C1 在溶剂（丙酮、丁醇和乙醇）和 COD 去除方面发挥了最重要的作用。C2 的群落结构在高 OLR 的第 3 阶段与 C1 相似，但在低 OLR 的第 1-2 阶段与 C3 和 C4 相似。C2 这种群落变化与其在第 3 阶段增加的 COD 和溶剂去除作用一致。在第 3 阶段从 C1 到 C4 的群落演替过程中，厚壁菌门（特别是 ABRB07 和 ABRB10 菌属）和甲烷微菌属丰度降低，而拟杆菌门和甲烷胞菌属成为优势菌属。因此，ABRB07 可能与甲烷微菌属和/或产乙酸菌（ABRB10）一起成为降解溶剂的关键物种。
