Department of Parasitology, Inje University College of Medicine, Busan 614-735, South Korea.
Acta Trop. 2011 Oct-Nov;120(1-2):40-5. doi: 10.1016/j.actatropica.2011.05.006. Epub 2011 Jun 6.
The rapid, accurate diagnosis of Plasmodium spp. is essential for the effective control of malaria, especially in asymptomatic infections. In this study, we developed a sensitive, genus-specific, real-time quantitative PCR assay. It was compared with the microscopic examination of Giemsa-stained blood smears and two different molecular diagnostic techniques: nested PCR and multiplex PCR. For the effective quantitative detection of malaria parasites, all reagents were designed with a lyophilized format in one tube. Plasmodium was detected successfully in all 112 clinically suspected malaria patients, including 32 individuals with low parasitemia (1-100 parasites/μl). The sensitivity threshold was 0.2 parasites/μl and no PCR-positive reaction occurred when malaria parasites were not present. This may be a useful method for detecting malaria parasites in endemic areas.
快速、准确地诊断疟原虫属对于有效控制疟疾至关重要，特别是在无症状感染的情况下。在本研究中，我们开发了一种敏感的、属特异性的实时定量 PCR 检测方法。该方法与吉姆萨染色血涂片的显微镜检查和两种不同的分子诊断技术（巢式 PCR 和多重 PCR）进行了比较。为了有效地定量检测疟原虫，所有试剂都以冻干形式装在一个管中。该方法成功地检测了 112 例临床疑似疟疾患者中的所有样本，包括 32 例低疟原虫血症（1-100 个寄生虫/μl）患者。该方法的灵敏度阈值为 0.2 个寄生虫/μl，当不存在疟原虫时，不会发生 PCR 阳性反应。这可能是一种在流行地区检测疟原虫的有用方法。
