Angielski S, Kuchta G, Redlak M, Szczepanska-Konkel M
Department of Clinical Biochemistry, Medical Academy, Gdansk, Poland.
Biochem Biophys Res Commun. 1990 Jul 31;170(2):596-602. doi: 10.1016/0006-291x(90)92133-k.
Atrial natriuretic factor administered in the large dose did not change glomerular filtration rate, but it was diuretic in low-sodium rats. In response to ANF, excretion of c-GMP was decreased in low-sodium rats in comparison with normal-sodium stimulated c-GMP accumulation in isolated glomeruli was more diminished in low- than normal sodium rats. These results indicate that attenuated glomerular responses to ANF in low-sodium rats might be due to increase of plasma Angiotensin II (Ang II) level, which increases intracellular Ca++ concentration. Theophylline can potentiate the renal response to ANF. We suggest that Ca(++)-activated c-GMP phosphodiesterase plays a major role in the regulation of intracellular accumulation of c-GMP in glomeruli exposed to ANF.
大剂量给予心房利钠因子并未改变肾小球滤过率，但对低钠大鼠有利尿作用。与正常钠饮食大鼠相比，低钠大鼠对心房利钠因子的反应是c - GMP排泄减少，而低钠大鼠分离肾小球中对心房利钠因子刺激的c - GMP积累比正常钠饮食大鼠减少得更多。这些结果表明，低钠大鼠肾小球对心房利钠因子反应减弱可能是由于血浆血管紧张素II（Ang II）水平升高，后者增加了细胞内Ca++浓度。茶碱可增强肾脏对心房利钠因子的反应。我们认为，Ca(++)激活的c - GMP磷酸二酯酶在调节暴露于心房利钠因子的肾小球中c - GMP的细胞内积累中起主要作用。
