新型 HPV16 E6E7 融合基因突变体的转化活性。
Transforming activity of a novel mutant of HPV16 E6E7 fusion gene.
机构信息
College of Life Science and Bioengineering, Beijing University of Technology, China.
出版信息
Virol Sin. 2011 Jun;26(3):206-13. doi: 10.1007/s12250-011-3178-9. Epub 2011 Jun 12.
Abstract
An optimized recombinant HPV16 E6E7 fusion gene (HPV16 ofE6E7) was constructed according to codon usage for mammalian cell expression, and a mutant of HPV16 ofE6E7 fusion gene (HPV16 omfE6E7) was generated by site-directed mutagenesis at L57G, C113R for the E6 protein and C24G, E26G for the E7 protein for HPV16 ofE6E7 [patent pending (CN 101100672)]. The HPV16 omfE6E7 gene constructed in this work not only lost the transformation capability to NIH 3T3 cells and tumorigenicity in SCID mice, but also maintained very good stability and antigenicity. These results suggests that the HPV16 omfE6E7 gene should undergo further study for application as a safe antigen-specific therapeutic vaccine for HPV16-associated tumors.
摘要
根据哺乳动物细胞表达的密码子使用情况，构建了优化的 HPV16 E6E7 融合基因（HPV16 ofE6E7），并通过定点突变在 L57G、C113R 处生成 HPV16 ofE6E7 融合基因（HPV16 omfE6E7）突变体，用于 HPV16 的 E6 蛋白和 E7 蛋白 C24G、E26G [专利申请中（CN 101100672）]。本工作构建的 HPV16 omfE6E7 基因不仅丧失了转化 NIH 3T3 细胞的能力和在 SCID 小鼠中的致瘤性，而且保持了非常好的稳定性和抗原性。这些结果表明，HPV16 omfE6E7 基因应该进一步研究作为 HPV16 相关肿瘤的安全抗原特异性治疗疫苗的应用。
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