Nemecek G M, Ray K P, Butcher R W
J Biol Chem. 1979 Feb 10;254(3):598-601.
Short term incubation of WI-38 cultures with 5.7 micron prostaglandin E1 (PGE1) caused cyclic AMP phosphodiesterase activity in fibroblast homogenates to fall by 25 to 35% as compared to controls. The PGE1-induced decline in phosphodiesterase activity coincided with a rapid increase in intracellular cyclic AMP levels in response to the hormone and was rapidly reversed by washing the cultures free of the prostaglandin before homogenizing the cells. The effect of PGE1 on WI-38 phosphodiesterase activity was localized to the enzyme form(s) present in 27,000 times g supernatant fractions of cell homogenates. These data suggest that the pattern of cyclic AMP accumulation in WI-38 fibroblasts exposed to PGE1 may be related, at least in part, to decreased phosphodiesterase activity during hormone stimulation.
将WI-38细胞培养物与5.7微米的前列腺素E1（PGE1）进行短期孵育，结果显示，与对照组相比，成纤维细胞匀浆中的环磷酸腺苷磷酸二酯酶活性下降了25%至35%。PGE1诱导的磷酸二酯酶活性下降与细胞内环磷酸腺苷水平因该激素而迅速升高同时发生，并且在对细胞进行匀浆前，通过洗涤去除培养物中的前列腺素，该效应可迅速逆转。PGE1对WI-38磷酸二酯酶活性的影响定位于细胞匀浆27,000倍重力下上清液组分中存在的酶形式。这些数据表明，暴露于PGE1的WI-38成纤维细胞中环磷酸腺苷积累模式可能至少部分与激素刺激期间磷酸二酯酶活性降低有关。
