Wang Xiao-ning, Tao Qing, Feng Qin, Peng Jing-hua, Liu Ping, Hu Yi-yang
Institute of Liver Diseases, Shuguang Hospital, E-institute of Traditional Chinese Internal Medicine of Shanghai Municipal Education Commission, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong Xi Yi Jie He Xue Bao. 2011 Jun;9(6):651-7. doi: 10.3736/jcim20110612.
To investigate the effects of Yiguanjian Decoction, a compound traditional Chinese herbal medicine, on collagen metabolism of hepatic tissues in rats with carbon tetrachloride (CCl(4))-induced liver fibrosis.
Liver fibrosis was induced in rats by intraperitoneal injection of 50% CCl(4)-olive oil solution at a dose of 1 mL/kg body weight, twice per week for 9 consecutive weeks. Six rats were sacrificed for dynamic observation at the end of the 3rd and 6th week respectively, and the other rats were divided into 9-week untreated group and Yiguanjian Decoction group which was given Yiguanjian Decoction intragastrically in the subsequent 3-week modeling period. Another 6 rats were used as normal group. Rats in the normal group and 9-week untreated group were treated with distilled water. At the end of the 9th week, all rats were sacrificed, and their blood serum and liver tissue were collected for measuring hepatic histology and expressions of α-smooth muscle actin (α-SMA), tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, matrix metalloproteinase (MMP)-13, MMP-14, collagen type I (Col I), and activities of MMP-2 and -9.
Compared with the normal group, collagen fiber deposition, expressions of α-SMA, Col I, TIMP-1, TIMP-2, MMP-13 and MMP-14 and activities of MMP-2 and -9 in the liver tissues gradually increased in the untreated group (P<0.05, P<0.01). These changes were significantly suppressed by Yiguanjian Decoction.
Yiguanjian Decoction exerts inhibition on formation of CCl(4)-induced cirrhosis in rats. The therapeutic mechanism may be related to inhibiting hepatic stellate cell activation, collagen secretion, and promoting collagen fiber degradation.
探讨中药复方一贯煎对四氯化碳（CCl₄）诱导的大鼠肝纤维化肝组织胶原代谢的影响。
采用腹腔注射50% CCl₄-橄榄油溶液，剂量为1 mL/kg体重，每周2次，连续9周，诱导大鼠肝纤维化。分别于第3周和第6周结束时处死6只大鼠进行动态观察，其余大鼠分为9周未治疗组和一贯煎组，在随后3周的造模期内给予一贯煎灌胃。另取6只大鼠作为正常组。正常组和9周未治疗组大鼠给予蒸馏水。第9周结束时，处死所有大鼠，收集血清和肝组织，检测肝组织学及α-平滑肌肌动蛋白（α-SMA）、金属蛋白酶组织抑制剂（TIMP）-1、TIMP-2、基质金属蛋白酶（MMP）-13、MMP-14、Ⅰ型胶原（Col Ⅰ）的表达以及MMP-2和MMP-9的活性。
与正常组相比，未治疗组肝组织胶原纤维沉积、α-SMA、Col Ⅰ、TIMP-1、TIMP-2、MMP-13和MMP-14的表达以及MMP-2和MMP-9的活性逐渐增加（P<0.05，P<0.01）。一贯煎可显著抑制这些变化。
一贯煎对CCl₄诱导的大鼠肝硬化形成具有抑制作用。其治疗机制可能与抑制肝星状细胞活化、胶原分泌及促进胶原纤维降解有关。
