两种人巨核母细胞白血病细胞系中细胞内环状AMP增加剂对血栓调节蛋白表达的增强作用。

Enhanced expression of thrombomodulin by intracellular cyclic AMP-increasing agents in two human megakaryoblastic leukemia cell lines.

作者信息

Ito T, Ogura M, Morishita Y, Takamatsu J, Maruyama I, Yamamoto S, Ogawa K, Saito H

机构信息

First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

出版信息

Thromb Res. 1990 Jun 15;58(6):615-24. doi: 10.1016/0049-3848(90)90307-x.

DOI:10.1016/0049-3848(90)90307-x

PMID:2166972

Abstract

Functionally active thrombomodulin (TM) was expressed in human megakaryoblastic leukemia (MEG-01s) cells. We examined the effect of agents that increased the intracellular concentration of cAMP on the expression of TM by these cells. N6,O2-dibutyryl cAMP (dbcAMP) markedly enhanced TM antigen, activity, and mRNA level in MEG-01s cells. Other agents, 8-bromo-cAMP (8BrcAMP), forskolin, and prostaglandin E1 were also effective for the enhancement. Moreover, similar enhancement of TM by these agents was also observed in another human leukemia cell line, HEL, which has megakaryocytic markers. In contrast to the marked enhancement of TM expression by these agents, the expression of the other megakaryocytic markers including platelet glycoproteins IIb/IIIa, Ib, von Willebrand factor and beta-thromboglobulin was not stimulated in MEG-01s or HEL cells. These results suggest that expression of TM is rather specifically regulated by cAMP in human megakaryocytes.

摘要

功能性活性血栓调节蛋白（TM）在人巨核母细胞白血病（MEG - 01s）细胞中表达。我们研究了增加细胞内cAMP浓度的试剂对这些细胞TM表达的影响。N6，O2 - 二丁酰cAMP（dbcAMP）显著增强了MEG - 01s细胞中的TM抗原、活性和mRNA水平。其他试剂，8 - 溴 - cAMP（8BrcAMP）、福斯可林和前列腺素E1也有增强作用。此外，在另一种具有巨核细胞标志物的人白血病细胞系HEL中也观察到这些试剂对TM的类似增强作用。与这些试剂对TM表达的显著增强相反，在MEG - 01s或HEL细胞中，包括血小板糖蛋白IIb/IIIa、Ib、血管性血友病因子和β - 血小板球蛋白在内的其他巨核细胞标志物的表达未受到刺激。这些结果表明，在人巨核细胞中，TM的表达相当特异性地受cAMP调节。