Department of Advanced Skin Care, The University of Tokyo, Tokyo, Japan.
Biol Res Nurs. 2012 Jul;14(3):242-9. doi: 10.1177/1099800411410870. Epub 2011 Jun 13.
Obesity is recognized as a risk factor for delayed cutaneous wound healing. The authors hypothesized that the secretion of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) from subcutaneous adipose tissue correlates with disorder of the healing process in obese subjects. Findings from previous studies on the expression of MMPs and TIMPs in obese adipose tissue are inconsistent. Since these conflicting results could be due to the effect of several intrinsic factors, the authors conducted a simple in vitro experiment to clarify the change in profile of MMPs and TIMPs in excessively matured adipocytes. The authors cultured the induced adipocytes under conditions of high or low glucose and with or without insulin supplementation. Oil red O staining and its dye extraction assay revealed excessive lipid accumulation in high glucose and insulin-supplemented adipocytes. Additionally, there was altered expression of adipokines, similar to the change in adipose tissue in obese subjects. Under these conditions, the expression/activity of MMP8 was promoted and the expression of MMP3 and TIMP3 was inhibited. Further studies to determine the effect of other obesity-related factors, such as insulin resistance, on MMPs and TIMPs are required.
肥胖被认为是延迟皮肤伤口愈合的一个风险因素。作者假设，来自皮下脂肪组织的基质金属蛋白酶（MMPs）及其组织抑制剂（TIMPs）的分泌与肥胖患者的愈合过程紊乱有关。先前关于肥胖脂肪组织中 MMPs 和 TIMPs 表达的研究结果不一致。由于这些相互矛盾的结果可能是由于几个内在因素的影响，作者进行了一项简单的体外实验，以阐明过度成熟脂肪细胞中 MMPs 和 TIMPs 谱的变化。作者在高葡萄糖和/或胰岛素补充的条件下培养诱导的脂肪细胞。油红 O 染色及其染料提取试验显示，高葡萄糖和胰岛素补充的脂肪细胞中脂质过度积累。此外，还存在类似肥胖患者脂肪组织的变化的脂肪细胞因子表达改变。在这些条件下，MMP8 的表达/活性增加，而 MMP3 和 TIMP3 的表达受到抑制。需要进一步研究确定其他与肥胖相关的因素，如胰岛素抵抗，对 MMPs 和 TIMPs 的影响。
