[两种产生牛白血病病毒的细胞系中的不同反式激活过程]
[Different transactivation processes in two bovine leukemia virus producing cell lines].
作者信息
Wagner H J, Blankenstein P, Bondzio A, Burkhardt H
机构信息
Sektion Tierproduktion und Veterinärmedizin der Humboldt-Universität zu Berlin, Wissenschafts-bereich Biochemie und Lehrstuhl Virologie.
出版信息
Arch Exp Veterinarmed. 1990;44(2):329-39.
Comparative studies were conducted through more than six months into quantitative of bovine leukaemia virus (BLV) antigen of FLC/BLV 44 and its FLC/BLV 44-4 subline by means of an enzyme immuno-assay (EIA), using monoclonal antibodies against gp51 and p24. Synthesis of gp51 (factors of two to six) and of p24 (factor of two) by FLC/BLV 44 was clearly higher than that by FLC/BLV 44-4. The transactivation status in either line was determined by transfer of the beta-galactosidase indicator organ under transcription control of BLV-LTR (in pBLV beta Gal plasmid). Transient experiments showed beta-galactosidase activity in the FLC/BLV 44 to be clearly higher than that in subline FLC/BLV 44-4. There is obviously in both cell lines a close correlation between intensity of BLV antigen synthesis and transactivation processes.
通过酶免疫测定法（EIA），使用针对gp51和p24的单克隆抗体，对FLC/BLV 44及其FLC/BLV 44-4亚系的牛白血病病毒（BLV）抗原进行了为期六个多月的定量比较研究。FLC/BLV 44合成的gp51（2至6倍因子）和p24（2倍因子）明显高于FLC/BLV 44-4。通过在BLV-LTR（pBLVβGal质粒中）转录控制下转移β-半乳糖苷酶指示基因来确定任一细胞系中的反式激活状态。瞬时实验表明，FLC/BLV 44中的β-半乳糖苷酶活性明显高于FLC/BLV 44-4亚系。在两种细胞系中，BLV抗原合成强度与反式激活过程之间显然存在密切相关性。
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