Department of Public Health, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan.
Biol Trace Elem Res. 2011 Dec;144(1-3):388-95. doi: 10.1007/s12011-011-9107-9. Epub 2011 Jun 14.
Several studies show the consistent results of the decrease in plasma or serum selenium (Se) after surgery, and the change is suggested to be a negative acute phase response of Se to the surgical inflammation. Plasma glutathione peroxidase (GPx), which is included in the acute phase response proteins, is a selenoenzyme. However, previous studies failed to show any changes in GPx activity before and after surgery. In the present study, we investigated the Se- and selenoenzyme responses that accompany the acute inflammatory reactions during and following major surgery. Patients who underwent elective total knee arthroplasty surgery due to knee osteoarthritis at the Department of Orthopaedic Surgery at Gunma University Hospital in Japan were studied. The plasma Se concentration was determined, and the activity of plasma GPx was measured. C-reactive protein (CRP), albumin, blood urea nitrogen (BUN), and white blood cell (WBC) count were also analysed. Increases in the inflammatory biomarkers of CRP and WBC showed inflammatory reactions with the surgery. A significant increase in plasma GPx activity (p < 0.05) and decreases in the plasma Se concentration (p < 0.05) and in serum albumin (p < 0.05) after surgery were observed. Since albumin is a Se-containing protein and represents a negative acute phase protein that provides amino acids for the production of other series of acute phase proteins, the present results suggest that there is a redistribution of plasma Se to GPx that occurs as an acute phase response, and the source of Se for GPx could be, at least partly, from albumin.
几项研究表明，手术后血浆或血清硒（Se）含量持续下降，这种变化被认为是 Se 对手术炎症的急性负面反应。血浆谷胱甘肽过氧化物酶（GPx）是急性反应蛋白之一，属于含硒酶。然而，先前的研究未能显示手术前后 GPx 活性有任何变化。在本研究中，我们研究了伴随重大手术期间和之后急性炎症反应的 Se 和硒酶反应。研究对象为日本群马大学医院骨科因膝骨关节炎行择期全膝关节置换术的患者。测定血浆 Se 浓度，测量血浆 GPx 活性。还分析了 C-反应蛋白（CRP）、白蛋白、血尿素氮（BUN）和白细胞（WBC）计数。CRP 和 WBC 等炎症生物标志物的增加表明存在与手术相关的炎症反应。术后观察到血浆 GPx 活性显著增加（p<0.05），血浆 Se 浓度（p<0.05）和血清白蛋白（p<0.05）降低。由于白蛋白是一种含 Se 蛋白，代表一种负急性相蛋白，可为其他系列急性相蛋白的产生提供氨基酸，因此本研究结果表明，作为急性相反应，血浆 Se 向 GPx 发生再分配，而 GPx 的 Se 来源至少部分来自白蛋白。
