[结直肠癌中ZIC1和KLOTHO基因启动子的DNA甲基化及其临床病理意义]
[DNA methylation of ZIC1 and KLOTHO gene promoters in colorectal carcinomas and its clinicopathological significance].
作者信息
Gan Li-hong, Pan Jie, Chen Shu-jie, Zhong Jing, Wang Liang-jing
机构信息
Department of Gastroenterology, College of Medicine, Zhejiang University, Hangzhou 310009, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2011 May;40(3):309-14. doi: 10.3785/j.issn.1008-9292.2011.03.014.
OBJECTIVE
To determine DNA methylation status of ZIC1 and KLOTHO gene in colorectal carcinomas and its relationship with clinicopathological features of patients.
METHODS
The mRNA expression of ZIC1 and KLOTHO genes in colorectal carcinomas was detected by real-time quantitative RT-PCR, and the promoter methylation status was detected by methylation specific PCR (MSP). The relationship of ZIC1 and KLOTHO methylation status with clinicopathological features of colorectal carcinoma was analyzed.
RESULT
The mRNA expression levels of ZIC1 and KLOTHO genes were significantly down-regulated in tumor tissues when compared to adjacent nontumor tissues (P<0.001). ZIC1 and KLOTHO methylation was detected in 80.0%(20/25) and 76.0%(19/25) of colorectal tumor tissues, respectively, and the both positive rate was 64.0%(16/25).
CONCLUSION
The down-regulated expression of ZIC1 and KLOTHO in colorectal carcinoma may relate to promoter methylation. The detection of methylation of ZIC1 and KLOTHO gene potentially provides biomarkers for diagnosis of colorectal carcinoma.
目的
确定结直肠癌中ZIC1和KLOTHO基因的DNA甲基化状态及其与患者临床病理特征的关系。
方法
采用实时定量逆转录聚合酶链反应（RT-PCR）检测结直肠癌组织中ZIC1和KLOTHO基因的mRNA表达，并采用甲基化特异性PCR（MSP）检测启动子甲基化状态。分析ZIC1和KLOTHO甲基化状态与结直肠癌临床病理特征的关系。
结果
与癌旁非肿瘤组织相比，肿瘤组织中ZIC1和KLOTHO基因的mRNA表达水平显著下调（P<0.001）。结直肠癌组织中ZIC1和KLOTHO甲基化检出率分别为80.0%（20/25）和76.0%（19/25），两者均阳性率为64.0%（16/25）。
结论
结直肠癌中ZIC1和KLOTHO表达下调可能与启动子甲基化有关。ZIC1和KLOTHO基因甲基化检测可能为结直肠癌诊断提供生物标志物。
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