免疫组织化学分析在前列腺腺癌中的 ezrin-radixin-moesin 结合磷蛋白 50。
Immunohistochemical analysis of ezrin-radixin-moesin-binding phosphoprotein 50 in prostatic adenocarcinoma.
机构信息
University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
出版信息
BMC Urol. 2011 Jun 14;11:12. doi: 10.1186/1471-2490-11-12.
BACKGROUND
Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is an adapter protein which has been shown to play an active role in a wide variety of cellular processes, including interactions with proteins related to both tumor suppression and oncogenesis. Here we use immunohistochemistry to evaluate EBP50's expression in normal donor prostate (NDP), benign prostatic hyperplasia (BPH), high grade prostatic intraepithelial neoplasia (HGPIN), normal tissue adjacent to prostatic adenocarcinoma (NAC), primary prostatic adenocarcinoma (PCa), and metastatic prostatic adenocarcinoma (Mets).
METHODS
Tissue microarrays were immunohistochemically stained for EBP50, with the staining intensities quantified using automated image analysis software. The data were statistically analyzed using one-way ANOVA with subsequent Tukey tests for multiple comparisons. Eleven cases of NDP, 37 cases of NAC, 15 cases of BPH, 35 cases of HGPIN, 103 cases of PCa, and 36 cases of Mets were analyzed in the microarrays.
RESULTS
Specimens of PCa and Mets had the lowest absolute staining for EBP50. Mets staining was significantly lower than NDP (p = 0.027), BPH (p = 0.012), NAC (p < 0.001), HGPIN (p < 0.001), and PCa (p = 0.006). Additionally, HGPIN staining was significantly higher than NAC (p < 0.009) and PCa (p < 0.001).
CONCLUSIONS
To our knowledge, this represents the first study comparing the immunohistochemical profiles of EBP50 in PCa and Mets to specimens of HGPIN, BPH, NDP, and NAC and suggests that EBP50 expression is decreased in Mets. Given that PCa also had significantly higher expression than Mets, future studies are warranted to assess EBP50's potential as a prognostic biomarker for prostate cancer.
背景
埃兹蛋白-根蛋白-膜突蛋白结合磷蛋白 50（EBP50）是一种衔接蛋白，它在多种细胞过程中发挥着积极的作用，包括与肿瘤抑制和致癌相关蛋白的相互作用。在这里，我们使用免疫组织化学方法来评估 EBP50 在正常供体前列腺（NDP）、良性前列腺增生（BPH）、高级别前列腺上皮内瘤变（HGPIN）、前列腺腺癌旁正常组织（NAC）、原发性前列腺腺癌（PCa）和转移性前列腺腺癌（Mets）中的表达。
方法
使用组织微阵列进行 EBP50 的免疫组织化学染色，使用自动化图像分析软件对染色强度进行定量。使用单因素方差分析和随后的 Tukey 检验进行多重比较对数据进行统计学分析。在微阵列中分析了 11 例 NDP、37 例 NAC、15 例 BPH、35 例 HGPIN、103 例 PCa 和 36 例 Mets。
结果
PCa 和 Mets 标本的 EBP50 绝对染色最低。Mets 的染色明显低于 NDP（p = 0.027）、BPH（p = 0.012）、NAC（p < 0.001）、HGPIN（p < 0.001）和 PCa（p = 0.006）。此外，HGPIN 的染色明显高于 NAC（p < 0.009）和 PCa（p < 0.001）。
结论
据我们所知，这是第一项比较 EBP50 在 PCa 和 Mets 与 HGPIN、BPH、NDP 和 NAC 标本的免疫组织化学特征的研究，并表明 EBP50 的表达在 Mets 中降低。鉴于 PCa 的表达也明显高于 Mets，因此有必要进一步研究评估 EBP50 作为前列腺癌预后生物标志物的潜力。
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