Ackermann U, Geering K
Institut de Pharmacologie de l'Université, Lausanne, Switzerland.
FEBS Lett. 1990 Aug 20;269(1):105-8. doi: 10.1016/0014-5793(90)81130-g.
In this study, we have followed the fate of newly synthesized alpha- and beta-subunits of Na,K-ATPase in Xenopus oocytes injected with alpha and/or beta cRNA to examine whether assembly of the two subunits is needed for a correct folding and/or for intracellular transport of Na,K-ATPase. Our data indicate that (1) assembly of alpha- and beta-subunits occurs at the level of the ER, (2) beta-subunits are needed for the newly synthesized alpha-subunit to adopt a stable configuration and (3) alpha- and beta-subunits mutually depend on each other to be transported out of the ER.
在本研究中，我们追踪了非洲爪蟾卵母细胞中钠钾ATP酶新合成的α和β亚基的去向，这些卵母细胞被注射了α和/或β互补RNA（cRNA），以检查两个亚基的组装对于钠钾ATP酶的正确折叠和/或细胞内运输是否必要。我们的数据表明：（1）α和β亚基的组装发生在内质网水平；（2）新合成的α亚基需要β亚基才能形成稳定的构象；（3）α和β亚基相互依赖才能被转运出内质网。
