Pincemail J, Defraigne J O, Franssen C, Defechereux T, Canivet J L, Philippart C, Meurisse M
Laboratory of Biochemistry and Radiobiology, University of Liège, Belgium.
Free Radic Res Commun. 1990;9(3-6):181-6. doi: 10.3109/10715769009145675.
By using alpha-phenyl N-tert-butyl nitrone (PBN) as spin trap molecule and the electron paramagnetic resonance (EPR) technique, we obtained the first direct evidence of in vivo intervention of free radicals during an ischemia (50 minutes) reperfusion phenomenon in kidney of an intact rabbit. An EPR signal (triplet of doublets) characterized by coupling constants aN = 14.75-15 G and aH beta = 2.5-3 G was detected in blood samples. The signal was consistent with a nitroxyl-radical adduct resulting from the spin trapping by PBN of either oxygen-or carbon-centered radicals. Control experiments indicated that the EPR signal was not due to a toxic effect of the spin trap molecule.
通过使用α-苯基N-叔丁基硝酮（PBN）作为自旋捕获分子，并采用电子顺磁共振（EPR）技术，我们首次获得了自由基在完整兔肾脏缺血（50分钟）再灌注现象期间进行体内干预的直接证据。在血样中检测到一个EPR信号（双峰三重态），其特征在于耦合常数aN = 14.75 - 15 G和aHβ = 2.5 - 3 G。该信号与由PBN对以氧或碳为中心的自由基进行自旋捕获所产生的硝酰自由基加合物一致。对照实验表明，EPR信号并非由于自旋捕获分子的毒性作用所致。
