模拟细胞膜的支撑脂质膜的制备与动态图案化

Preparation and dynamic patterning of supported lipid membranes mimicking cell membranes.

作者信息

Kaufmann Stefan, Kumar Karthik, Reimhult Erik

出版信息

Methods Mol Biol. 2011;751:453-63. doi: 10.1007/978-1-61779-151-2_28.

Abstract

In this chapter, we describe standardized protocols for the self-assembly of supported lipid bilayers (SLBs) from liposomes with lipid compositions mimicking eukaryote and prokaryote cell membranes. Such SLBs can also contain lipids with polymeric and glycosylated headgroups. Furthermore, we present protocols on how to manipulate the adsorption and desorption of membranes on indium tin oxide (ITO) electrodes, which allows for the creation of patterned and in situ regenerated SLB arrays that can be used to study electrochemically mediated membrane processes in a microarray format.

摘要

在本章中，我们描述了用于从脂质体自组装支撑脂质双层（SLB）的标准化方案，这些脂质体的脂质组成模拟真核生物和原核生物细胞膜。此类SLB还可包含带有聚合物和糖基化头基的脂质。此外，我们还介绍了如何控制膜在氧化铟锡（ITO）电极上的吸附和解吸的方案，这使得能够创建可用于以微阵列形式研究电化学介导的膜过程的图案化和原位再生SLB阵列。