Noy Aleksandr, Artyukhin Alexander B, Huang Shih-Chieh, Martinez Julio A, Misra Nipun
School of Natural Sciences, University of California, Merced, CA, USA.
Methods Mol Biol. 2011;751:533-52. doi: 10.1007/978-1-61779-151-2_33.
Biological molecules perform a sophisticated array of transport and signaling functions that rival anything that the modern electronics industry can create. Incorporating such building blocks into nanoelectronic devices could enable new generations of electronic circuits that use biomimetics to perform complicated tasks. Such types of circuits could ultimately blur the interface between living biological organisms and synthetic structures. Our laboratory has recently developed a versatile and flexible platform for integrating ion channels and pumps into single-walled carbon nanotube (SWNT) and silicon nanowire (SiNW) transistor devices, in which membrane proteins are embedded in a lipid bilayer shell covering the nanotube or nanowire component. In this chapter, we provide details for the fabrication of these devices and outline procedures for incorporating biological molecules into them. In addition, we also provide several examples of the use of these devices to couple biological transport to electronic signaling.
生物分子执行一系列复杂的运输和信号功能，这些功能可与现代电子工业所能创造的任何东西相媲美。将此类构建模块纳入纳米电子器件中，有望实现新一代利用仿生学来执行复杂任务的电子电路。这类电路最终可能会模糊活生物体与合成结构之间的界面。我们实验室最近开发了一个通用且灵活的平台，用于将离子通道和泵整合到单壁碳纳米管（SWNT）和硅纳米线（SiNW）晶体管器件中，其中膜蛋白嵌入覆盖纳米管或纳米线组件的脂质双层壳中。在本章中，我们详细介绍了这些器件的制造过程，并概述了将生物分子纳入其中的程序。此外，我们还提供了几个使用这些器件将生物运输与电子信号耦合的示例。
