Department of Biotechnology, Indian Institute of Technology Roorkee, Uttarakhand, India.
J Agric Food Chem. 2011 Jul 13;59(13):7465-73. doi: 10.1021/jf2008277. Epub 2011 Jun 15.
A coupled in vitro digestion/Caco-2 model was employed to assess iron bioavailability from wheat Aegilops derivatives selected for high iron and protein contents. The iron content in wheat genotypes used in this study correlated to a great extent with both protein (r = 0.80) and phytate (r = 0.68) contents. The iron bioavailability was based on Caco-2 cell ferritin formation from cooked digests of these derivatives (relative to WL711 control) and correlated positively with dialyzable iron (r = 0.63) and total iron content (r = 0.38) but not with the phytate content. The apparently decreased phytate/iron molar ratios, however, correlated negatively (r = -0.42) with the iron bioavailability, justifying the utilization of these parameters in biofortification programs. Iron bioavailability in the derivatives increased up to 1.5-fold, corresponding to a 1.5-2.2-fold increase observed in iron content over control. These data suggest that biofortification for iron proportionately leading to higher iron bioavailability will be the most feasible and cost-effective approach to combat micronutrient deficiency.
采用体外消化/Caco-2 模型来评估从选择富含铁和蛋白质的小麦 Aegeilops 衍生品种中获得的铁的生物利用度。本研究中使用的小麦基因型的铁含量与蛋白质（r = 0.80）和植酸（r = 0.68）含量密切相关。铁的生物利用度基于这些衍生品种的熟化消化物在 Caco-2 细胞中的铁蛋白形成（相对于 WL711 对照），并与可透析铁（r = 0.63）和总铁含量（r = 0.38）呈正相关，但与植酸含量无关。然而，明显降低的植酸盐/铁摩尔比与铁的生物利用度呈负相关（r = -0.42），这证明了在生物强化计划中利用这些参数的合理性。衍生品种中的铁生物利用度增加了 1.5 倍，与对照相比，铁含量增加了 1.5-2.2 倍。这些数据表明，为了提高铁的生物利用度而进行生物强化将是对抗微量营养素缺乏最可行和最具成本效益的方法。
