Pereira Mirella Leite, de Oliveira Hermogenes David, de Oliveira Jose Tadeu Abreu, Gifoni Juliana Menezes, Rocha Raquel de Oliveira, de Sousa Daniele de Oliveira Bezerra, Vasconcelos Ilka Maria
Universidade Federal do Ceara, Departamento de Bioquimica e Biologia Molecular, 60440-990, Fortaleza, CE, Brazil.
Protein Pept Lett. 2011 Nov;18(11):1078-85. doi: 10.2174/092986611797200959.
Moringa oleifera Lam. is a perennial multipurpose tree that has been successfully used in folk medicine to cure several inflammatory processes. The aim of this study was to purify and characterize a chitin-binding protein from Moringa oleifera seeds, named Mo-CBP4, and evaluate its antinociceptive and anti-inflammatory effects in vivo. The protein was purified by affinity chromatography on chitin followed by ion exchange chromatography. Acetic acid-induced abdominal constrictions assay was used for the antinociceptive and anti-inflammatory activity assessments. Mo-CBP4 is a glycoprotein (2.9% neutral carbohydrate) composed of two protein subunits with apparent molecular masses of 28 and 18 kDa (9 kDa in the presence of reducing agent). The intraperitoneal injection of Mo-CBP4 (3.5 and 10 mg/kg) into mice 30 min before acetic acid administration potently and significantly reduced the occurrence of abdominal writhing in a dose dependent manner by 44.7% and 100%, respectively. In addition, the oral administration of the protein (10 mg/kg) resulted in 18% and 52.8% reductions in abdominal writhing when given 30 and 60 min prior to acetic acid administration, respectively. Mo-CBP4, when administered by intraperitoneal route, also caused a significant and dose-dependent inhibition of peritoneal capillary permeability induced by acid acetic and significantly inhibited leukocyte accumulation in the peritoneal cavity. In conclusion, this pioneering study describes that the chitin-binding protein Mo-CBP4, from M. oleifera seeds, exhibits anti-inflammatory and antinociceptive properties and scientifically supports the use of this multipurpose tree in folk medicine.
辣木是一种多年生多用途树木，已成功应用于民间医学治疗多种炎症过程。本研究的目的是纯化和鉴定一种从辣木种子中提取的几丁质结合蛋白，命名为Mo-CBP4，并在体内评估其抗伤害感受和抗炎作用。该蛋白通过几丁质亲和层析，随后进行离子交换层析进行纯化。采用醋酸诱导的腹部收缩试验评估抗伤害感受和抗炎活性。Mo-CBP4是一种糖蛋白（2.9%中性碳水化合物），由两个蛋白质亚基组成，表观分子量分别为28 kDa和18 kDa（在还原剂存在下为9 kDa）。在给予醋酸前30分钟，向小鼠腹腔注射Mo-CBP4（3.5和10 mg/kg），能有效且显著地以剂量依赖方式减少腹部扭体的发生，分别减少44.7%和100%。此外，在给予醋酸前30分钟和60分钟口服该蛋白（10 mg/kg），腹部扭体分别减少18%和52.8%。当通过腹腔途径给药时，Mo-CBP4还能显著且剂量依赖性地抑制醋酸诱导的腹膜毛细血管通透性，并显著抑制腹腔内白细胞积聚。总之，这项开创性研究表明，来自辣木种子的几丁质结合蛋白Mo-CBP4具有抗炎和抗伤害感受特性，并从科学上支持了这种多用途树木在民间医学中的应用。
