鉴定口腔拟杆菌 T14V SrtC1 活性所需的 srtC1 转录起始位点和催化必需残基。
Identification of the srtC1 transcription start site and catalytically essential residues required for Actinomyces oris T14V SrtC1 activity.
机构信息
Microbiology Branch, US Army Dental and Trauma Research Detachment, Institute of Surgical Research, Fort Sam Houston, TX, USA.
出版信息
FEMS Microbiol Lett. 2011 Sep;322(2):115-22. doi: 10.1111/j.1574-6968.2011.02338.x. Epub 2011 Jul 27.
In Actinomyces oris T14V, sortase SrtC1 mediates the assembly of type 1 fimbriae. We analyzed the effects of the conserved residues (H184, H204, F213, Y236, L263, T265, C266 and R275) on the SrtC1 activity by site-directed mutagenesis. We identified three essential conserved residues (H204, Y236 and C266) that are critical for the assembly of type 1 fimbriae in this organism. rapid amplification of cDNA ends analyses and reverse transcriptase-PCR results indicate that srtC1 was transcribed together with the putative adhesin gene fimQ and major structural subunit gene fimP as a single polycistronic mRNA.
在口腔放线菌 T14V 中，Sortase SrtC1 介导了 1 型菌毛的组装。我们通过定点突变分析了保守残基（H184、H204、F213、Y236、L263、T265、C266 和 R275）对 SrtC1 活性的影响。我们确定了三个关键的保守残基（H204、Y236 和 C266），它们对该生物体中 1 型菌毛的组装至关重要。快速扩增 cDNA 末端分析和逆转录-PCR 结果表明，srtC1 与假定的黏附素基因 fimQ 和主要结构亚基基因 fimP 一起转录为单个多顺反子 mRNA。
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