Chen Xin, Hsueh Hain Ann, Werblin Frank S
Department of Molecular and Cell Biology, University of California, Berkeley, USA.
Vis Neurosci. 2011 May;28(3):193-204. doi: 10.1017/S0952523811000137.
We measured the spatial and temporal properties of GABAergic and glycinergic inhibition to amacrine cells in the whole-mount rabbit retina. The amacrine cells were parsed into two morphological classes: narrow-field cells with processes spreading less than 200 μm and wide-field cells with processes extending more than 300 μm. The inhibition was also parsed into two types: sustained glycine and transient GABA. Narrow-field amacrine cells receive 1) very transient GABAergic inhibition with a fast onset latency of 140 ± 16 ms decaying to 30% of the peak level within 208 ± 27 ms elicited broadly over a lateral distance of up to 1500 μm and 2) sustained glycinergic inhibition with a medium onset latency of 286 ± 23 ms that was elicited over a spatial area often broader than the processes of the narrow-field amacrine cells. Wide-field amacrine cells received sustained glycinergic inhibition but no broad transient GABAergic inhibition. Surprisingly, neither of these amacrine cell classes received sustained local GABAergic inhibition, commonly found in an earlier study of ganglion cells.
我们测量了全层兔视网膜中向无长突细胞的γ-氨基丁酸能和甘氨酸能抑制的空间和时间特性。无长突细胞被分为两种形态类型：突起扩展小于200μm的窄场细胞和突起延伸超过300μm的宽场细胞。抑制也分为两种类型：持续的甘氨酸抑制和短暂的γ-氨基丁酸抑制。窄场无长突细胞接受1）非常短暂的γ-氨基丁酸能抑制，起始潜伏期快，为140±16毫秒，在208±27毫秒内衰减至峰值水平的30%，在横向距离达1500μm的范围内广泛引发；2）持续的甘氨酸能抑制，起始潜伏期中等，为286±23毫秒，在一个通常比窄场无长突细胞的突起更宽的空间区域引发。宽场无长突细胞接受持续的甘氨酸能抑制，但没有广泛的短暂γ-氨基丁酸能抑制。令人惊讶的是，这些无长突细胞类型均未接受在早期对神经节细胞的研究中常见的持续局部γ-氨基丁酸能抑制。
