Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Hum Pathol. 2011 Dec;42(12):1960-4. doi: 10.1016/j.humpath.2011.02.019. Epub 2011 Jun 14.
Mortality from melanoma, the deadliest of skin cancers, continues to increase in all age groups. A small number of melanomas spontaneously regress. In vitro studies suggest a role for the natural killer cell in effecting regression. In this study, the goal was to determine if natural killer cells are preferentially involved in the cytotoxic response in regressing lesions. Forty-two cases were selected: nevi with regression, nonregressing melanoma with brisk inflammation, and regressing melanoma. Sections were stained with hematoxylin and eosin and immunostained for CD8, CD56, and T-cell intracytoplasmic antigen 1. Numbers of total lymphocytes, CD8-positive lymphocytes, and T-cell intracytoplasmic antigen 1-positive lymphocytes did not differ among the 3 populations or based on location. CD56 positivity was significantly different among the 3 populations. Regressing melanomas showed the greatest CD56 activity, followed by regressing nevi, whereas inflamed, nonregressing melanomas showed the least. CD56(+) lymphocytes were mostly counted in areas of early regression. The natural killer cell could plausibly play a role in the occurrence of regression as a cytotoxic effector cell or as a mediator of the cytotoxic mechanism.
黑色素瘤是最致命的皮肤癌之一，其死亡率在所有年龄段都在持续上升。少数黑色素瘤会自发消退。体外研究表明自然杀伤细胞在消退中起作用。在这项研究中，目的是确定自然杀伤细胞是否优先参与消退病变中的细胞毒性反应。选择了 42 例病例：有消退的痣、有活跃炎症的非消退黑色素瘤和消退的黑色素瘤。用苏木精和伊红染色，并免疫染色 CD8、CD56 和 T 细胞胞浆内抗原 1。3 组或根据位置的总淋巴细胞、CD8 阳性淋巴细胞和 T 细胞胞浆内抗原 1 阳性淋巴细胞数量没有差异。CD56 阳性在 3 组之间有显著差异。消退的黑色素瘤显示出最强的 CD56 活性，其次是消退的痣，而有炎症的非消退黑色素瘤显示出最弱的活性。CD56(+)淋巴细胞主要计数在早期消退的区域。自然杀伤细胞作为细胞毒性效应细胞或细胞毒性机制的介质，可能在消退的发生中发挥作用。
