Araki S, Kawahara Y, Fukuzaki H, Takai Y
Department of Internal Medicine, 1st Division, Kobe University School of Medicine, Japan.
Atherosclerosis. 1990 Jul;83(1):29-34. doi: 10.1016/0021-9150(90)90127-5.
Whole blood serum (WBS) rapidly induced the phospholipase C-mediated hydrolysis of phosphoinositides and subsequently stimulated DNA synthesis in cultured rabbit vascular smooth muscle cells (VSMCs). Ketanserin, a serotonin (S2) receptor antagonist, markedly inhibited the WBS-induced phospholipase C reaction and DNA synthesis. Serotonin by itself had a weak mitogenic activity for VSMCs, but this vasoconstrictor markedly stimulated the platelet-derived growth factor- and epidermal growth factor-induced DNA synthesis. The stimulatory effect of serotonin on the growth factor-induced DNA synthesis was inhibited by ketanserin. The amount of serotonin contained in WBS was sufficient to induce the phospholipase C reaction and stimulate the growth factor-induced DNA synthesis. These results indicate that serotonin plays a major role in the WBS-induced phospholipase C-mediated hydrolysis of phosphoinositides and DNA synthesis in rabbit VSMCs and suggest that serotonin may act as an important growth regulator for VSMCs in addition to acting as a vasoconstrictor.
全血血清（WBS）能迅速诱导磷脂酶C介导的磷酸肌醇水解，随后刺激培养的兔血管平滑肌细胞（VSMC）中的DNA合成。酮色林是一种5-羟色胺（S2）受体拮抗剂，能显著抑制WBS诱导的磷脂酶C反应和DNA合成。5-羟色胺自身对VSMC具有较弱的促有丝分裂活性，但这种血管收缩剂能显著刺激血小板衍生生长因子和表皮生长因子诱导的DNA合成。5-羟色胺对生长因子诱导的DNA合成的刺激作用被酮色林抑制。WBS中所含的5-羟色胺量足以诱导磷脂酶C反应并刺激生长因子诱导的DNA合成。这些结果表明，5-羟色胺在兔VSMC中WBS诱导的磷脂酶C介导的磷酸肌醇水解和DNA合成中起主要作用，并提示5-羟色胺除作为血管收缩剂外，可能还作为VSMC的重要生长调节因子发挥作用。
