Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland.
Photosynth Res. 2011 Sep;108(2-3):241-5. doi: 10.1007/s11120-011-9662-0. Epub 2011 Jun 16.
Slr0006 is one of the Synechocystis sp. PCC 6803 proteins strongly induced under carbon limiting conditions. Slr0006 has no predicted transmembrane helices or signal peptide sequence, yet it was exclusively recovered in the membrane fraction of Synechocystis, when the cells were broken in isolation buffers which contain divalent cations and are generally used for photosynthesis studies. Even subsequent washing of the membranes with high salt or various detergents did not release Slr0006, indicating strong binding of the Slr0006 protein to the membranes. Further, DNAse or RNAse treatment did not disturb the tight binding of Slr0006 protein to the membranes. Nevertheless, when the cells were broken in the absence of divalent cations, Slr0006 remained completely soluble. Binding of the Slr0006 to the membrane could not be properly reconstituted if the cations were added after breaking the cells in the absence of divalent ions. This unusual phenomenon has to be considered in identification and localization of other yet uncharacterized cyanobacterial proteins.
Slr0006 是集胞藻 PCC6803 中一种在碳限制条件下强烈诱导的蛋白。Slr0006 没有预测的跨膜螺旋或信号肽序列，但当细胞在含有二价阳离子的分离缓冲液中破碎时，它仅在集胞藻的膜部分被回收，而这些分离缓冲液通常用于光合作用研究。即使随后用高盐或各种洗涤剂洗涤膜也不能释放 Slr0006，这表明 Slr0006 蛋白与膜的结合非常牢固。此外，DNA 酶或 RNA 酶处理不会干扰 Slr0006 蛋白与膜的紧密结合。然而，当细胞在没有二价阳离子的情况下破碎时，Slr0006 仍然完全可溶。如果在没有二价离子的情况下破碎细胞后再加入阳离子，则无法正确重建 Slr0006 与膜的结合。在鉴定和定位其他尚未表征的蓝藻蛋白时，必须考虑这种不寻常的现象。
