WM Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
Expert Rev Proteomics. 2011 Jun;8(3):317-23. doi: 10.1586/epr.11.24.
Protein chemical derivatization has emerged as an invaluable bioanalytical approach in mass spectrometry-based proteomics with nearly unlimited potential. To date, derivatization strategies in proteomics have primarily focused on improving mass spectral identification and relative quantification of proteins, as well as increasing enrichment yield from complex mixtures. However, there is a great opportunity to develop and exploit front-end chemical processes to enhance the ability to detect low-abundant peptides and proteins for a large number of applications. The content of this article focuses on improvements in targeted, mass spectrometry-based proteomic strategies, achieved by taking advantage of the mechanism of ESI through the use of hydrophobic chemical derivatization.
蛋白质化学衍生已成为基于质谱的蛋白质组学中一种非常有价值的生物分析方法，具有几乎无限的潜力。迄今为止，蛋白质组学中的衍生化策略主要集中在提高蛋白质的质谱鉴定和相对定量，以及从复杂混合物中增加富集产量。然而，有很大的机会开发和利用前端化学过程来提高检测低丰度肽和蛋白质的能力，以满足大量的应用需求。本文的内容主要集中在通过利用 ESI 的机制，通过使用疏水性化学衍生化，在基于靶向、质谱的蛋白质组学策略方面的改进。
