Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Am J Rhinol Allergy. 2011 Mar-Apr;25(2):131-6. doi: 10.2500/ajra.2011.25.3576.
Data on the molecular processes involved in nasal mucosa wound healing after radiofrequency tissue ablation (RTA) of the inferior nasal turbinate (INT) are missing. This study was designed to examine tissue expression of fibronectin, collagen III, CD68, and matrix metalloproteinase (MMP) 9 in the INT (ventral) after RTA in sheep.
An experimental randomized controlled study was performed. Seventeen INTs (ventral) of nine sheep were used. RTA was applied in 12 INTs. Turbinate samples were studied 1, 3, and 8 weeks postoperatively (4 samples/time point) and in five control INTs (without surgery). Besides hematoxylin and eosin staining, immunostaining was done for MMP-9, collagen III, fibronectin, and CD68. A quantitative grading ranging between 0 (no immunoreactivity at all) and 100% (profuse immunoreactivity) was performed by a blinded senior pathologist. Comparisons between groups were performed using Mann-Whitney U test and Kruskal-Wallis one-way ANOVA. Spearman's rho correlation coefficients were calculated between histological and/or immunohistological variables.
At week 8, fibronectin (p = 0.025), collagen III (p = 0.004), and MMP-9 (p < 0.001) immunoreactivity was significantly higher than controls, while immunoreactivity for CD68 was higher, although not significantly (p = 0.114) compared with controls. Strong correlations have been found between mucosal vascularization and interstitial space volume (r = 0.776), interstitial space volume and epithelial cell necrosis (r = 0.730), and CD68 immunostaining and epithelial cell necrosis (r = 0.784).
Given their high tissue concentrations after RTA application, fibronectin, collagen III, CD68, and MMP-9 deserve further study as candidate modulators of the INT wound-healing process.
关于下鼻甲（INT）经射频组织消融（RTA）后鼻黏膜伤口愈合所涉及的分子过程的数据尚不清楚。本研究旨在检查绵羊 RTA 后 INT（腹侧）组织中纤维连接蛋白、III 型胶原、CD68 和基质金属蛋白酶（MMP）9 的表达。
进行了一项实验性随机对照研究。使用了 9 只绵羊的 17 个 INT（腹侧）。12 个 INT 应用 RTA。术后 1、3 和 8 周（每个时间点 4 个样本）研究鼻甲样本，并在 5 个对照 INT（无手术）中研究。除苏木精和伊红染色外，还进行了 MMP-9、III 型胶原、纤维连接蛋白和 CD68 的免疫染色。一位经验丰富的病理学家通过盲法对 0（无免疫反应）到 100%（丰富免疫反应）的范围进行定量分级。使用 Mann-Whitney U 检验和 Kruskal-Wallis 单向方差分析进行组间比较。计算组织学和/或免疫组织化学变量之间的 Spearman 相关系数。
第 8 周时，纤维连接蛋白（p = 0.025）、III 型胶原（p = 0.004）和 MMP-9（p < 0.001）的免疫反应明显高于对照组，而 CD68 的免疫反应虽然较高，但无统计学意义（p = 0.114）与对照组相比。黏膜血管化与间质空间体积之间存在很强的相关性（r = 0.776），间质空间体积与上皮细胞坏死之间存在很强的相关性（r = 0.730），CD68 免疫染色与上皮细胞坏死之间存在很强的相关性（r = 0.784）。
鉴于 RTA 应用后这些物质在组织中的浓度较高，纤维连接蛋白、III 型胶原、CD68 和 MMP-9 作为 INT 伤口愈合过程的候选调节剂值得进一步研究。
