Graduate School of Engineering Science, Osaka University, Machikaneyama 1-3, Toyonaka, Osaka 560-8531, Japan.
Biosens Bioelectron. 2011 Aug 15;26(12):4819-22. doi: 10.1016/j.bios.2011.05.035. Epub 2011 May 30.
This study describes a sensitivity-amplified detection method in a replacement-free electrodeless quartz-crystal microbalance (QCM) biosensor. A sandwich assay is proposed for detecting C-reactive proteins (CRP), where the biotinated second anti-CRP antibody is weighted by streptavidin for sensitivity amplification. Because the first CRP antibody was immobilized nonspecifically on naked quartz surfaces, the sandwich assay was repeated using the same sensor chip, making possible the replacement-free assay. The mass-amplified sandwich assay detected a CRP solution of 0.1 ng/ml. A methodology for determining the molecular mass of the injected protein is also proposed.
本研究描述了一种免更换的无电极石英晶体微天平（QCM）生物传感器中的灵敏度增强检测方法。提出了一种夹心检测方法用于检测 C 反应蛋白（CRP），其中生物素化的第二种抗 CRP 抗体通过链霉亲和素进行加权以实现灵敏度放大。由于第一种 CRP 抗体非特异性地固定在裸露的石英表面上，因此可以使用相同的传感器芯片重复进行夹心检测，从而实现免更换检测。这种质量放大的夹心检测法可以检测到浓度为 0.1ng/ml 的 CRP 溶液。本文还提出了一种用于确定注入蛋白质分子量的方法。
